In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33.1 kDa and the accession number is Q00941.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | 1. Immobilized Mouse GM-CSF R alpha, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test). 2. Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay. |
产品描述 | In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33.1 kDa and the accession number is Q00941. |
种属 | Mouse |
表达系统 | HEK293 |
标签 | C-His |
蛋白编号 | Q00941 |
别名 | CSF2RAX, CSF2RX, CD116, CSF2RAY, GM-CSF-R-α, GMCSFR-α, GMCSFR-alpha, CSF2RY, CSF2RA, GMR-alpha, GMCSFR, CSF2R, CDw116, GM-CSF-R-alpha, GMR-α, GMR, SMDP4, GM-CSF R α Protein |
蛋白构建 | Recombinant Mouse GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu30-Pro327.[Accession |Q00941] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | The protein has a predicted MW of 33.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
GM-CSFR alpha Protein, Mouse, Recombinant (His) CSF2RAX CSF2RX SMDP-4 CD116 CSF2RAY GM-CSF-R-α GMCSFR-α GMCSFR-alpha CSF2RY CD 116 CSF2RA GMR-alpha GMCSFR SMDP 4 CSF2R CDw116 GM-CSF-R-alpha GMR-α GMR SMDP4 GM-CSF R α Protein CD-116 recombinant recombinant-proteins proteins protein