GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated

In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 37.7 kDa and the accession number is P15509-1.

规格	价格/CNY		数量
100 μg	￥ 5,180	5日内发货
500 μg	￥ 20,700	5日内发货
1 mg	￥ 34,600	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 5,180

5日内发货

500 μg

￥ 20,700

5日内发货

1 mg

￥ 34,600

5日内发货

生物活性

1. Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 7.7ng/ml determined by ELISA. 2. Biotinylated Human GM-CSF R alpha, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay.

产品描述

种属	Human
表达系统	HEK293
标签	C-His-Avi
蛋白编号	P15509-1
别名	GM-CSF-R-α, GMR-α, GMR-alpha, GM-CSF-R-alpha, CSF2R, GMCSFR-alpha, CSF2RAY, GMCSFR-α, GMR, CSF2RAX, CSF2RX, CDw116, SMDP4, GM-CSF R α Protein, CSF2RY, 集落刺激因子, GMCSFR, CSF2RA, CD116
蛋白构建	Recombinant Biotinylated Human GM-CSF R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu23-Gly320.[Accession \|P15509-1]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.