Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,660 | 5日内发货 | ||
500 μg | ¥ 10,600 | 5日内发货 | ||
1 mg | ¥ 17,800 | 5日内发货 |
生物活性 | 1. Immobilized Human Activin A, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA. 2. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A. |
产品描述 | Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. |
种属 | Human |
表达系统 | HEK293 |
标签 | C-His |
蛋白编号 | O95633-1 |
别名 | FSTL3, FLRGFSRP, Follistatin-like 3, FLRG |
蛋白构建 | Recombinant Human FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met27-Val263.[Accession |O95633-1] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD. |
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FSTL3 Protein, Human, Recombinant (His) FSTL3 FLRGFSRP Follistatin-like 3 FSTL-3 FSTL 3 FLRG recombinant recombinant-proteins proteins protein