FGFR2 Protein, Human, Recombinant (aa 400-821, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.7 kDa and the accession number is P21802-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 3,170 | 5日内发货 |
生物活性 | 1. The specific activity was determined to be 28 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic with a linear range of 15.6-250 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized recombinant recombinant human FGFR2 (aa 400-821) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF basic with a linear range of 0.16-1.25 μg/ml. |
产品描述 | FGFR2 Protein, Human, Recombinant (aa 400-821, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.7 kDa and the accession number is P21802-1. |
种属 | Human |
表达系统 | Baculovirus-Insect Cells |
标签 | His,GST |
蛋白编号 | P21802-1 |
别名 | CFD1, ECT1, K-SAM, BEK, KGFR, CD332, JWS, BFR-1, fibroblast growth factor receptor 2, BBDS, TK14, CEK3, TK25 |
蛋白构建 | A DNA sequence encoding the human FGFR2 (NP_000132.3) cytoplasmic domain (Met 400-Thr 821) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 75.7 kDa (predicted) |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glyPlease contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
研究背景 | FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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FGFR2 Protein, Human, Recombinant (aa 400-821, His & GST) CFD1 CEK 3 ECT1 CD 332 K-SAM TK-25 BEK KGFR TK 14 CD332 JWS CD-332 ECT 1 BFR1 CFD-1 BFR-1 TK-14 fibroblast growth factor receptor 2 ECT-1 BBDS TK14 CEK3 CEK-3 TK25 TK 25 CFD 1 BFR 1 recombinant recombinant-proteins proteins protein