FCGRT & B2M Heterodimer Protein, Human, Recombinant (His)

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 cells.

规格	价格/CNY		数量
100 μg	￥ 4,680	现货
200 μg	￥ 8,260	5日内发货
500 μg	￥ 17,440	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 4,680

现货

200 μg

￥ 8,260

5日内发货

500 μg

￥ 17,440

5日内发货

生物活性

1. Measured by its ability to bind human IgG1 in a functional ELISA.
2. Loaded Human FcRn recombinant protein on His1K Biosensor, can bind IgG1 with an affinity constant of 0.11 µM as determined in a BLI assay.
3. Loaded Human FcRn recombinant protein on His1k Biosensor, can bind IgG4 Fc with an affinity constant of 0.08 µM as determined in a BLI assay.
4. Captured Human FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 µM as determined in an SPR assay (Biacore T200).
5. Captured Human FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 µM as determined in an SPR assay (Biacore T200).
6. The purity of Human FCGRT & B2M Heterodimer Protein is more than 90% and the molecular weight of this protein is around 45-53 kDa verified by SEC-MALS.
7. Loaded Recombinant Human FCGRT & B2M Heterodimer Protein, His Tag on His1K Biosensor, can bind Anti-Erbb2 Antibody, human IgG1 with an affinity constant of 11.1nM as determined in BLI assay (QC tested).

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产品描述

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 cells.

种属	Human
表达系统	HEK293
标签	His
蛋白编号	P55899-1&P61769
别名	Fc fragment of IgG, receptor, transporter, alpha, alpha-chain, α-chain, Fc fragment of IgG, receptor, transporter, α, FCRN
蛋白构建	A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
蛋白纯度	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
分子量	43.5 kDa (predicted)

内毒素	< 1.0 EU per μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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FCGRT & B2M Heterodimer Protein, Human, Recombinant (His)

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