Ephrin B3/EFNB3 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.5 kDa and the accession number is O35393.
生物活性 | 1.Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EFNB3-His at 10 μg/mL (100μL/well) can bind biotinylated mouse EPHB3-His, the EC50 of biotinylated mouse EPHB3-His 0.02-0.4 μg/mL. |
产品描述 | Ephrin B3/EFNB3 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.5 kDa and the accession number is O35393. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | O35393 |
别名 | ephrin-B3, EFL-6, ELF-3, NLERK-2, LERK-8, Elk-L3, Epl8 |
蛋白构建 | The Mouse Efnb3 (NP_031937.1) (Met1-Ala227) was expressed with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 95 % as determined by SDS-PAGE. |
分子量 | 23.5 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons. |
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Ephrin B3/EFNB3 Protein, Mouse, Recombinant (His) LERK 8 ephrin-B3 ELF3 EFL-6 EFL6 NLERK2 ELF 3 ELF-3 NLERK-2 EFL 6 LERK8 NLERK 2 LERK-8 Elk-L3 Epl8 recombinant recombinant-proteins proteins protein