EGFRVIII Protein, Human, Recombinant (His & Avi), Biotinylated

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

规格	价格/CNY		数量
100 μg	￥ 5,180	5日内发货
500 μg	￥ 20,700	5日内发货
1 mg	￥ 34,600	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 5,180

5日内发货

500 μg

￥ 20,700

5日内发货

1 mg

￥ 34,600

5日内发货

生物活性

Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 56.1ng/ml determined by ELISA.

产品描述

种属	Human
表达系统	HEK293
标签	C-His-Avi
蛋白编号	NP_001333870.1
别名	EC 2.7.10.1, PIG61, NISBD2, HER1, mENA, LEGFR, ErbB, EC 2.7.10, ERBB1, EGFR
蛋白构建	Recombinant Biotinylated Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu25-Ser378.[Accession \|NP_001333870.1]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.