Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,180 | 5日内发货 | ||
500 μg | ¥ 20,700 | 5日内发货 | ||
1 mg | ¥ 34,600 | 5日内发货 |
生物活性 | Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA. |
产品描述 | Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc-Avi |
蛋白编号 | O94907 |
别名 | hDkk-1, SK, Dkk1, Dickkopf-related 1, Dickkopf-1, Dkk-1, hDkk1 |
蛋白构建 | Met178-His266 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 38.69 kDa (predicted). Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAc, 150 mM NaCl (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. |
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DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated hDkk-1 SK Dkk1 Dickkopf-related 1 Dickkopf-1 Dkk-1 hDkk1 recombinant recombinant-proteins proteins protein