CTLA-4 Protein, Human, Recombinant (GST) is expressed in HEK293 mammalian cells with C-GST tag. The predicted molecular weight is 40-55 KDa and the accession number is P16410.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 540 | 5日内发货 | ||
50 μg | ¥ 1,170 | 5日内发货 | ||
500 μg | ¥ 5,250 | 5日内发货 | ||
1 mg | ¥ 7,510 | 5日内发货 |
生物活性 | Immobilized Mouse B7-1-Fc at 5μg/ml (100 μl/well) can bind Human CTLA-4-GST*.*: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant Human CTLA-4-GST is 1.17 ug/ml. (Regularly tested) |
产品描述 | CTLA-4 Protein, Human, Recombinant (GST) is expressed in HEK293 mammalian cells with C-GST tag. The predicted molecular weight is 40-55 KDa and the accession number is P16410. |
种属 | Human |
表达系统 | Human Cells |
标签 | C-GST |
蛋白编号 | P16410 |
别名 | Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4 |
氨基酸序列 | Lys36-Asp161 |
蛋白构建 | Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a GST tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 40-55 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4. |
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CTLA-4 Protein, Human, Recombinant (GST) Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4 recombinant recombinant-proteins proteins protein