CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
200 μg | ¥ 3,820 | 现货 |
生物活性 | 1 μg = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃ |
产品描述 | CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells. |
种属 | Streptococcus pyogenes |
表达系统 | Baculovirus-Insect Cells |
标签 | His |
蛋白编号 | Q99ZW2 |
别名 | SpyCas9 |
蛋白构建 | A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus. |
蛋白纯度 |
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
分子量 | Approxiamtely 163 kDa |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0. 5 mM PMSF, 0. 1 mM EDTA, 0. 5 mM TCEP. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
Solution. It is shipped out with blue ice. |
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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) SpyCas9 recombinant recombinant-proteins proteins protein