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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)

CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)

产品编号 TMPY-05346
别名: SpyCas9

CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells.

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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His)
规格 价格/CNY 货期 数量
200 μg ¥ 3,820 现货
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
重组蛋白限时优惠
产品目录号及名称: CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) (TMPY-05346)
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生物活性
技术参数
产品性质
生物活性 1 μg = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃
产品描述 CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus-Insect cells.
种属 Streptococcus pyogenes
表达系统 Baculovirus-Insect Cells
标签 His
蛋白编号 Q99ZW2
别名 SpyCas9
蛋白构建 A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.
蛋白纯度 ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

TargetMol TargetMol

分子量 Approxiamtely 163 kDa
内毒素 < 1.0 EU per μg protein as determined by the LAL method.
缓冲液 Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0. 5 mM PMSF, 0. 1 mM EDTA, 0. 5 mM TCEP. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法 A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
存储

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

运输方式

Solution. It is shipped out with blue ice.

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Keywords

CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) SpyCas9 recombinant recombinant-proteins proteins protein

 

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