CNTF Protein, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 22.9 kDa and the accession number is P20294.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 1,050 | 5日内发货 | ||
100 μg | ¥ 3,930 | 5日内发货 | ||
1 mg | ¥ 22,900 | 5日内发货 |
生物活性 | 1. Immobilized rat CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) with a linear range of 1.56-50.0 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-100 ng/ml. |
产品描述 | CNTF Protein, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 22.9 kDa and the accession number is P20294. |
种属 | Rat |
表达系统 | E. coli |
标签 | Tag Free |
蛋白编号 | P20294 |
别名 | ciliary neurotrophic factor |
蛋白构建 | A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified. |
蛋白纯度 | > 92 % as determined by SDS-PAGE |
分子量 | 22.9 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from sterile 50mM Tris, 0. 1M NaCl, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors. |
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CNTF Protein, Rat, Recombinant ciliary neurotrophic factor recombinant recombinant-proteins proteins protein