Claudin-9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.44 kDa and the accession number is O95484.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 15,400 | 5日内发货 | ||
100 μg | ¥ 54,000 | 5日内发货 |
生物活性 | 1. Immobilized Human CLDN9-Nanodisc Protein(His & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL,100 μL/well) can bind Anti-Human Claudin9 Antibody, Human IgG1, the EC50 is 10-60 ng/mL. 2.Loaded Biotinylated Recombinant Human CLDN9-Nanodisc Protein, His & AVI Tag on SA Biosensor, can bind Anti-CLDN9 antibody with an affinity constant of 11.15 nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested). |
产品描述 | Claudin-9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.44 kDa and the accession number is O95484. |
种属 | Human |
表达系统 | HEK293 |
标签 | His,AVI |
蛋白编号 | O95484 |
蛋白构建 | A DNA sequence encoding the human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment. |
蛋白纯度 | >80% as determined by SDS-PAGE. |
分子量 | 22.44 kDa (predicted) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Supplied as 0. 2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
存储 |
Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
It is supplied and shipped as Solution with dry ice. |
研究背景 | Claudin-9 (CLDN9) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and a cytoplasmic C tail. CLDN9 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It forms heterotypic interactions with other claudins to create cation-selective channels in the kidney and may contribute to the maintenance of alveolar barrier function in the lung. Deficiency is shown to be associated with autosomal recessive deafness, DFNB116. CLDN9 expression has been shown to be upregulated in certain types of cancer, such as endometrial cancer and hepatocellular carcinoma, and its knockdown has been found to reduce cell proliferation and migration in vitro. |
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recombinant recombinant-proteins proteins protein