Claudin-6 Protein, Human, Recombinant (His & FLAG)

Claudin-6 (CLDN6) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and intracellular N and C tails. CLDN6 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It regulates the paracellular flux of cations in the kidney through forming heterotypic strands with other claudins and has a role in maintaining the lung epithelial barrier function. The CLDN6 gene is adjacent to another family member, CLDN9, on chromosome 16. Certain CLDN6-positive cancers, encompassing ovarian, endometrial, and testicular malignancies, have been shown to have their proliferation and invasiveness suppressed upon CLDN6 silencing.

规格	价格/CNY		货期	数量
20 μg	￥ 15,400	5日内发货
100 μg	￥ 54,000	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 15,400

5日内发货

100 μg

￥ 54,000

5日内发货

生物活性

1.Loaded Anti-CLDN6 antibody on ProA Biosensor, can bind Human CLDN6 Protein with an affinity constant of 0.536nM as determined in BLI assay (Routinely tested). 2.Captured Anti-Human CLDN6 antibody on CM5 Chip via anti-human IgG Fc antibody, can bind Recombinant Human CLDN6-Nanodisc Protein (His & Flag Tag) with an affinity constant of 7.03 nM as determined in a SPR assay (Routinely tested).

产品描述

种属	Human
表达系统	HEK293
标签	His,FLAG
蛋白编号	P56747
别名	claudin 6
蛋白构建	A DNA sequence encoding the human CLDN6 (P56747) (Met1-Thr184) was expressed, with a Flag tag followed by a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
蛋白纯度	≥ 90 % as determined by SDS-PAGE
分子量	21.87 kDa (predicted)

内毒素	< 1.0 EU per μg protein as determined by the LAL method.
缓冲液	Supplied as 0. 2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
存储	Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	It is supplied and shipped as Solution with dry ice.
研究背景	Claudin-6 (CLDN6) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and intracellular N and C tails. CLDN6 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It regulates the paracellular flux of cations in the kidney through forming heterotypic strands with other claudins and has a role in maintaining the lung epithelial barrier function. The CLDN6 gene is adjacent to another family member, CLDN9, on chromosome 16. Certain CLDN6-positive cancers, encompassing ovarian, endometrial, and testicular malignancies, have been shown to have their proliferation and invasiveness suppressed upon CLDN6 silencing.