CD28 Protein, Human/Cynomolgus, Recombinant (His)

T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

规格	价格/CNY		数量
10 μg	￥ 550	现货
50 μg	￥ 1,670	5日内发货
500 μg	￥ 13,000	5日内发货
1 mg	￥ 19,500	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 550

现货

50 μg

￥ 1,670

5日内发货

500 μg

￥ 13,000

5日内发货

1 mg

￥ 19,500

5日内发货

生物活性

1. Loaded Anti-Human CD28 mAb-Fc on Protein A Biosensor, can bind Human/Cynomolgus CD28-His with an affinity constant of 0.77 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human/Cynomolgus CD28-His at 2μg/ml (100 μl/well) can bind Anti-CD28 mAb The ED50 of Anti-CD28 mAb is 15.3 ng/ml. (Regularly tested)

TargetMol Bioactive

产品描述

种属	Human,Cynomolgus
表达系统	Human Cells
标签	N-6His
蛋白编号	P10747
别名	CD28 antigen, CD28 molecule, T-cell-specific surface glycoprotein CD28, CD28, Tp44
氨基酸序列	Asn19-Pro152
蛋白构建	Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	30-40 KDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
复溶方法	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
存储	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输方式	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
研究背景	T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

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CD28 Protein, Human/Cynomolgus, Recombinant (His)

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