CD20 Protein, Human, Recombinant (His & Flag)

CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.

规格	价格/CNY		数量
10 μg	￥ 2,360	5日内发货
50 μg	￥ 7,110	5日内发货
500 μg	￥ 44,500	5日内发货
1 mg	￥ 67,300	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 2,360

5日内发货

50 μg

￥ 7,110

5日内发货

500 μg

￥ 44,500

5日内发货

1 mg

￥ 67,300

5日内发货

生物活性

Loaded Anti-Human CD20 mAb-Fc on AHC Biosensor, can bind Human CD20-His-Flag with an affinity constant of 1.3 pM as determined in BLI assay. (Regularly tested)

产品描述

种属	Human
表达系统	Human Cells
标签	C-6His-Flag
蛋白编号	P11836
别名	CD20, Membrane-spanning 4-domains subfamily A member 1, B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Bp35, MS4A1, B-lymphocyte antigen CD20
氨基酸序列	Met1-Pro297
蛋白构建	Recombinant Human B-lymphocyte antigen CD20 is produced by our Mammalian expression system and the target gene encoding Met1-Pro297 is expressed with a 6His, Flag tag at the C-terminus.
蛋白纯度	Greater than 80% as determined by reducing SDS-PAGE. (QC verified)
分子量	34-40 kDa, reducing conditions

内毒素	Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
缓冲液	Supplied as a 0.2 μm filtered solution of 50mM HEPES-Na, 150mM NaCl, 0.02%DDM, 0.004%CHS, 5%Glycerol, pH7.5.
存储	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
运输方式	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
研究背景	CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.

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CD20 Protein, Human, Recombinant (His & Flag)

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