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Carboxypeptidase E Protein, Human, Recombinant (His)

Carboxypeptidase E Protein, Human, Recombinant (His)

产品编号 TMPY-01141
别名: carboxypeptidase E

Carboxypeptidase E Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.4 kDa and the accession number is A0A384N679.

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Carboxypeptidase E Protein, Human, Recombinant (His)
规格 价格/CNY 货期 数量
50 μg ¥ 3,820 5日内发货
千万补贴 助力科研
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产品目录号及名称: Carboxypeptidase E Protein, Human, Recombinant (His) (TMPY-01141)
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生物活性
技术参数
产品性质
参考文献
生物活性 Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmoles/min/μg.
产品描述 Carboxypeptidase E Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.4 kDa and the accession number is A0A384N679.
种属 Human
表达系统 HEK293
标签 His
蛋白编号 A0A384N679
别名 carboxypeptidase E
蛋白构建 A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was fused with a polyhistidine tag at the C-terminus.
蛋白纯度 > 90 % as determined by SDS-PAGE
分子量 49.4 kDa (predicted)
内毒素 < 1.0 EU per μg of the protein as determined by the LAL method
缓冲液 Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法 A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

研究背景 Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

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Keywords

Carboxypeptidase E Protein, Human, Recombinant (His) carboxypeptidase E recombinant recombinant-proteins proteins protein

 

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