CAN F 4 Protein, Canine, Recombinant (His)

Dog dander is an important cause of respiratory allergy but its content of allergenic components is still incompletely known. The size and the amino acid composition of the ligand-binding pocket indicate that Can f 4 is capable of binding only relatively small hydrophobic molecules which are different from those that Can f 2 is able to bind. The crystal structure of Can f 4 contained both monomeric and dimeric forms of the allergen, suggesting that Can f 4 is able to form transient (weak) dimers. The existence of transient dimers in solution was confirmed by use of native mass spectrometry. The dimeric structure of Can f 4 is formed when the ends of four β-strands are packed against the same strands from the second monomer.

规格	价格/CNY		货期	数量
250 μg	￥ 5,100	5日内发货

规格

价格/CNY

货期

数量

250 μg

￥ 5,100

5日内发货

生物活性

Testing in progress

产品描述

种属	Canine
表达系统	HEK293
标签	His
蛋白编号	D7PBH4
蛋白构建	A DNA sequence encoding the canine Canf4 (NP_001177855.1) (Met1-Glu174) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE.
分子量	19.1 kDa (predicted)

内毒素	< 1.0 EU per μg protein as determined by the LAL method.
缓冲液	Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0. 02 % TWeen 80, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
复溶方法	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Dog dander is an important cause of respiratory allergy but its content of allergenic components is still incompletely known. The size and the amino acid composition of the ligand-binding pocket indicate that Can f 4 is capable of binding only relatively small hydrophobic molecules which are different from those that Can f 2 is able to bind. The crystal structure of Can f 4 contained both monomeric and dimeric forms of the allergen, suggesting that Can f 4 is able to form transient (weak) dimers. The existence of transient dimers in solution was confirmed by use of native mass spectrometry. The dimeric structure of Can f 4 is formed when the ends of four β-strands are packed against the same strands from the second monomer.

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复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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CAN F 4 Protein, Canine, Recombinant (His)

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