Bovine coronavirus (strain OK-0514) Spike glycoprotein (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 37.2 kDa and the accession number is Q9QAQ8.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,340 | 20日内发货 | ||
100 μg | ¥ 6,570 | 20日内发货 | ||
1 mg | ¥ 27,600 | 20日内发货 |
产品描述 | Bovine coronavirus (strain OK-0514) Spike glycoprotein (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 37.2 kDa and the accession number is Q9QAQ8. |
种属 | BCoV |
表达系统 | HEK293 Cells |
标签 | C-6xHis |
蛋白编号 | Q9QAQ8 |
别名 | Peplomer protein, S, S glycoprotein, E2, Spike glycoprotein |
氨基酸序列 | TVQPIADVYRRIPNLPDCNIEAWLNDKSVPSPLNWERKTFSNCNFNMSSLMSFIQADSFTCNNIDAAKIYGMCFSSITIDKFAIPNGRKVDLQLGNLGYLQSFNYRIDTTATSCQLYYNLPAANVSVSRFNPSIWNRRFGFTEQSVFKPQPAGVFTDHDVVYAQHCFKAPTNFCPCKLDGSLCVGSGSGIDAGYKNTGIGTCPAGTNYLTCHNAAQCGCLCTPDPITSKATGPYKCPQTKYLVGIGEHCSGLAIKSDYCGGNPCSCRPQAFLGWSVDSCLQGDRCNIFANFILHDVNSGTTCSTDLQKSNTDIILGVCVNY |
蛋白构建 | 314-634 aa |
蛋白纯度 | > 85% as determined by SDS-PAGE. |
分子量 | 37.2 kDa (predicted) |
缓冲液 | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. |
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Bovine coronavirus (strain OK-0514) Spike glycoprotein (His) Peplomer protein S S glycoprotein E2 Spike glycoprotein recombinant recombinant-proteins proteins protein