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Beta-Catenin Protein, Mouse, Recombinant (His & GST)

产品编号 TMPY-02985

别名: catenin β1, Bfc, Mesc, catenin beta 1, Catnb, β-Catenin

Beta-Catenin Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 113 kDa and the accession number is Q02248.

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Beta-Catenin Protein, Mouse, Recombinant (His & GST)

规格	价格/CNY		货期	数量
20 μg	￥ 2,270		5日内发货

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参考文献

生物活性	Testing in progress
产品描述	Beta-Catenin Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 113 kDa and the accession number is Q02248.

种属	Mouse
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	Q02248
别名	catenin β1, Bfc, Mesc, catenin beta 1, Catnb, β-Catenin
蛋白构建	The Mouse CTNNB1 (Q02248) (Met1-Leu781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 80 % as determined by SDS-PAGE
分子量	113 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

参考文献

1. Yang,et al.(2002) Linking β-catenin to androgen-signaling pathway. J Biol Chem. 277(13):11336-44. 2. Hino S,et al.(2005) Phosphorylation of β-Catenin by Cyclic AMP-Dependent Protein Kinase Stabilizes β-Catenin through Inhibition of Its Ubiquitination. Mol Cell Biol. 25(20):9063-72. 3. Liu X,et al.(2005) Rapid, Wnt-induced changes in GSK3beta associations that regulate beta-catenin stabilization are mediated by Galpha proteins. Curr Biol. 15(22):1989-97. 4. Kraus C,et al.(1994) Localization of the human β-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development. Genomics. 23(1):272-4.

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Keywords

Beta-Catenin Protein, Mouse, Recombinant (His & GST) catenin β1 Bfc Mesc catenin beta 1 Catnb catenin β 1 β-Catenin catenin b 1 recombinant recombinant-proteins proteins protein

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