ATP4B Protein, Human, Recombinant

The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.

规格	价格/CNY		数量
20 μg	￥ 2,300	20日内发货
100 μg	￥ 3,460	20日内发货
1 mg	￥ 11,700	20日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,300

20日内发货

100 μg

￥ 3,460

20日内发货

1 mg

￥ 11,700

20日内发货

产品描述

种属	Human
表达系统	E. coli
标签	NO-Tagged
蛋白编号	P51164
别名	Proton pump beta chain, Potassium-transporting ATPase subunit beta, ATP4B, Gastric H(+)/K(+) ATPase subunit beta
氨基酸序列	CLYVLMQTVDPYTPDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQESFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPRELGQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYEGKVEFKLKIEK Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白构建	58-291 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	26.6 kDa (predicted)

缓冲液	Tris-based buffer,50% glycerol
复溶方法	A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储	Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.

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ATP4B Protein, Human, Recombinant

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