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ASAH2 Protein, Mouse, Recombinant (His)

ASAH2 Protein, Mouse, Recombinant (His)

产品编号 TMPY-00963
别名: N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2, AI585898

ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

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ASAH2 Protein, Mouse, Recombinant (His)
规格 价格/CNY 货期 数量
50 μg ¥ 3,820 5日内发货
药物设计专题培训
千万补贴 助力科研
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产品目录号及名称: ASAH2 Protein, Mouse, Recombinant (His) (TMPY-00963)
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生物活性
技术参数
产品性质
参考文献
生物活性 Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is > 3,000 pmoles/min/μg.
产品描述 ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
种属 Mouse
表达系统 HEK293 Cells
标签 N-His
蛋白编号 B9EHG7
别名 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2, AI585898
蛋白构建 Lumenal domain (Thr 34-Thr 756) of Mouse ASAH2 (NP_061300.1) precursor
蛋白纯度 ≥ 95 % as determined by SDS-PAGE
分子量 82 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

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Keywords

ASAH2 Protein, Mouse, Recombinant (His) AI 585898 AI-585898 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 AI585898 recombinant recombinant-proteins proteins protein

 

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