ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,660 | 现货 | ||
500 μg | ¥ 10,600 | 5日内发货 | ||
1 mg | ¥ 17,800 | 5日内发货 |
产品描述 | ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib. |
种属 | Mouse |
表达系统 | E. coli |
标签 | N-His |
蛋白编号 | P07356 |
别名 | LIP2PAP-IV, Annexin II, LPC2DP36, LPC2D, CAL1H, Annexin A2, ANX2L4, ANX2, PAP-IV, p36, Lipocortin II, Chromobindin-8, ANXA2 |
蛋白构建 | Recombinant Mouse ANXA2 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ser2-Asp339.[Accession |P07356] |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | The protein has a predicted MW of 39.6 kDa same as Tris-Bis PAGE result. |
内毒素 | Less than 1EU per μg by the LAL method. |
缓冲液 | Lyophilized from 0.22μm filtered solution in 20mM MES, 150mM NaCl, 200mM L-arginine, 5mM DTT (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM L-arginine, 5mM DTT (pH 5.5). |
存储 |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
运输方式 |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
研究背景 | ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib. |
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ANXA2 Protein, Mouse, Recombinant (His) LIP2PAP-IV Annexin II ANX 2 LPC2DP36 ANXA-2 LPC2D CAL1H Annexin A2 ANXA 2 ANX2L4 ANX2 ANX-2 PAP-IV p36 Lipocortin II Chromobindin-8 ANXA2 recombinant recombinant-proteins proteins protein