AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 158 kDa. Accession number: P54619&Q9Y478&P54646
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 5,750 | 5日内发货 |
生物活性 | The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
产品描述 | AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 158 kDa. Accession number: P54619&Q9Y478&P54646 |
种属 | Human |
表达系统 | Baculovirus-Insect Cells |
标签 | His,GST |
蛋白编号 | P54619&Q9Y478&P54646 |
别名 | AMPK, AMPK(α2, β2, γ1) |
蛋白构建 | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 158 kDa (predicted) |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0. 5mM PMSF, 10% gly, 1mM GSH, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
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AMPK (G1/B2/A2) Heterotrimer Protein, Human, Recombinant (His & GST) AMPK, AMPK(α2, β2, γ1) recombinant recombinant-proteins proteins protein