5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 60-90 KDa and the accession number is Q13641.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,260 | 5日内发货 | ||
100 μg | ¥ 6,130 | 5日内发货 |
生物活性 | 1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Biotinylated Human 5T4 (C-Avi-6His) with an affinity constant of 9.29 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG Antibody (5G9) (Human IgG1) at 2μg/ml (100 μl/well) can bind Biotinylated Human TPBG-Avi-His. The ED50 of Human Biotinylated TPBG-Avi-His is 0.13 ng/ml. (Regularly tested) |
产品描述 | 5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 60-90 KDa and the accession number is Q13641. |
种属 | Human |
表达系统 | Human Cells |
标签 | C-Avi-6His |
蛋白编号 | Q13641 |
别名 | 5T4, M6P1, WAIF1, 5T4AG, TPBG |
氨基酸序列 | Ser32-Ser355 |
蛋白构建 | Biotinylated Recombinant Human Trophoblast Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a Avi, 6His tag at the C-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 60-90 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. |
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5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated 5T4 WAIF 1 WAIF-1 M6P1 WAIF1 5T4AG TPBG recombinant recombinant-proteins proteins protein