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Cell Counting Kit-8 (CCK-8)

Catalog No. C0005

产品描述:Cell Counting Kit-8 (CCK-8)试剂盒通过采用水溶性四唑盐 WST-8 (化学名:2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)实现快速检测,其原理是 WST-8在电子耦合试剂存在的情况下,可以被线粒体内的脱氢酶还原生成橙黄色的甲臜染料(formazan)。通过比色,可以动态地量化活细胞的数量,从而对细胞增殖或药物毒性进行检测。CCK-8溶液可直接加入到细胞中,不需要与其它组分预混,操作方便,检测准确。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
Cell Counting Kit-8 (CCK-8)
规格 价格/CNY 货期 数量
1 mL (100 tests) ¥ 168 现货
5 mL (500 tests) ¥ 419 现货
10 mL (1000 tests) ¥ 689 现货
30 mL (3000 tests) ¥ 1,596 现货
100 mL (10000 tests) ¥ 4,760 现货
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检测原理

Cell Counting Kit-8 (CCK-8)是一种氧化还原反应的指示剂,在电子载体1-Methoxy PMS存在的条件下,利用活细胞中的脱氢酶催化四唑盐WST-8生成甲臜染料,而且甲臜染料的生成量与活细胞的数量成线性关系。

检测方法 MTT法 XTT法 WST-1法 CCK8法
甲臜产物的水溶性
产品性状 粉末 2瓶溶液 溶液 1瓶溶液
使用方法 配成溶液后使用 现配现用 即开即用 即开即用
检测灵敏度 + ++ ++ +++
检测时间 较长 较短 较短 最短
检测波长 560~600nM 420~480nM 420~480nM 430~490nM
细胞毒性 高,细胞形态完全消失 很低,细胞形态不变 很低,细胞形态不变 很低,细胞形态不变
试剂稳定性 一般 较差 一般 很好
批量样品检测 可以 非常适合 非常适合 非常适合
便捷程度 一般 便捷 便捷 非常便捷

操作说明

1. 接种细胞悬液100μL 于96孔板,在细胞培养箱中(37°C, 5% CO2)孵育。

2. 取出需要检测的细胞,于细胞培养液中直接加入1/10体积的Cell Counting Kit-8 (CCK-8),充分混合,保证孔中颜色均一性,但不可有气泡产生。对96孔板,每 100µl培养液加入10µl检测试剂。

3. 继续于细胞培养箱中培养1-4小时。

4. 酶标仪读数之前,置于孔板于摇床振荡上约1min,确保孔板颜色均匀。

5. 用酶标仪读取450nm光吸收值,计算细胞活性。

细胞存活率=[(实验组-空白组) / (对照组-空白组)] × 100%

细胞抑制率=[(对照组-实验组) / (对照组-空白组)] × 100%

实验组:含细胞、培养基、CCK-8和药物;

对照组:含细胞、培养基、CCK-8,不含药物;

空白组:含培养基、CCK-8,不含细胞、药物。

6. 可选步骤:在100μl细胞中加入10 μl 3 % SDS 以终止反应,可在3天内读取数据。

注意事项

  • 使用前请充分混匀,避免反复冻融
  • 96孔板注意边缘效应,建议弃用周围一圈板孔,改加相同量的PBS。
  • 本产品只能用于研究,严禁用于人体。

产品优势

  • CCK-8的检测灵敏度高于其他方法,如MTT,XTT或WST-1;
  • 操作更简单,无需有机溶剂;
  • 对细胞无毒性;
  • 稳定的试剂,即开即用。

应用领域

  • 细胞增殖测定
  • 细胞活力检测
  • 细胞因子实验
  • 细胞毒性实验

储存条件

4℃避光可保存一年,-20℃避光可保存两年。

文献引用

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