Powder: -20°C for 3 years | In solvent: -80°C for 1 year
TAPI-2 is a broad-spectrum inhibitor of MMP (IC50: 20 μM), tumour necrosis factorα-converting enzyme (TACE) and a disintegrin and metalloproteinase (ADAM).
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | 待询 | 待询 | ||
50 mg | 待询 | 待询 | ||
100 mg | 待询 | 待询 |
产品描述 | TAPI-2 is a broad-spectrum inhibitor of MMP (IC50: 20 μM), tumour necrosis factorα-converting enzyme (TACE) and a disintegrin and metalloproteinase (ADAM). |
靶点活性 | MMP:20 μM, MMP:1.5 nM (Ki) |
体外活性 | TAPI-2 bounds to h-meprin with IC50 20 μM for h-meprin β subunit and 1.5 nM for h-meprin α subunit. Generally, h-meprin α is inhibited more strongly than the β subunit [1]. Without affecting ADAM17 expression, TAPI-2 dramatically decreases the protein levels of NICD and its downstream target HES-1 in both HCP-1 and HT29 cells. Moreover, treating cells with TAPI-2 significantly decreases the CSC phenotype by -50% in both CRC cell lines. The dose-dependent effects of TAPI-2 on the sphere formation and protein levels of NICD and HES-1 confirm that the concentration used (20 μM) is within the effective dose range of TAPI-2 (5–40 μM) [2]. |
别名 | TAPI-2 |
分子量 | 415.53 |
分子式 | C19H37N5O5 |
CAS No. | 187034-31-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 20 mg/mL (48.13 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4066 mL | 12.0328 mL | 24.0657 mL | 60.1641 mL |
5 mM | 0.4813 mL | 2.4066 mL | 4.8131 mL | 12.0328 mL | |
10 mM | 0.2407 mL | 1.2033 mL | 2.4066 mL | 6.0164 mL | |
20 mM | 0.1203 mL | 0.6016 mL | 1.2033 mL | 3.0082 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
TNF Protease Inhibitor 2 187034-31-7 Proteases/Proteasome MMP TAPI 2 TAPI-2 TAPI2 Inhibitor inhibitor inhibit