Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SecinH3 是cytohesins 的拮抗剂,是选择性细胞粘附素抑制剂。对于hCyh1、hCyh2、mCyh3、hCyh3、果蝇和yGea2-S7的IC50值分别为 5.4 μM、2.4 μM、5.4 μM、5.6 μM、5.6 μM 和 65 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 298 | 现货 | ||
5 mg | ¥ 690 | 现货 | ||
10 mg | ¥ 1,230 | 现货 | ||
25 mg | ¥ 2,370 | 现货 | ||
50 mg | ¥ 3,530 | 现货 | ||
100 mg | ¥ 4,970 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 713 | 现货 |
产品描述 | SecinH3 is selective cytohesin inhibitor. |
靶点活性 | Cyh3 (human):5.6 μM, Cyh1 (human):5.4 μM, Cyh3 (mouse):5.4 μM, Cyh2 (human):2.4 μM, Drosophila steppke:5.6 μM |
体外活性 | In HepG2 cells, SecinH3 inhibits insulin signaling and associated gene expression. [1] SecinH3 also markedly inhibits migration of preadipocyte 3T3-L1 cells. [2] In the A549 cells, SecinH3 results in reduced proliferation by indirectly attenuating EGFR activation, and causes a synergistic antiproliferative effect when used in combination with gefitinib. [3] |
体内活性 | In mice, SecinH3 increases the expression of gluconeogenic genes, reduces the expression of glycolytic, fatty acid and ketone body metabolism genes in the liver, reduces liver glycogen stores, and increases plasma insulin. [1] In mice bearing H460 xenografts, SecinH3 significantly retards tumor growth through its antiproliferative and pro-apoptotic effect. [3] |
激酶实验 | Aptamer displacement screen: A library of small molecules screened in duplicate from Comgenex (10000 molecules) in 386 well plates by fluorescence polarization at an excitation wavelength of 485 nm using read-out at 520 nm in a reaction volume of 50 μL at 37° C. The screening buffer is PBS, pH 7.5, 3 mM MgCl2, 100 nM fluorecein-labelled M69 aptamer, 1 μM cytohesin-1 Sec7 at 100 μM compound concentrations. |
细胞实验 | 3×103 cells per 96 well are seeded into a clear, flat bottom 96 well plate (TPP). After 24 h the cells are treated with the indicated concentrations of the inhibitors or solvent (final DMSO concentration 0.4%) in RPMI containing 50 ng/mL EGF or IGF-1, respectively. Medium is changed daily for 3 days and cell proliferation is analyzed with a MTT assas as described in the manufacturer's protocol using a Varioscan microplate reader. All assays are performed at least in triplicates. (Only for Reference) |
分子量 | 460.51 |
分子式 | C24H20N4O4S |
CAS No. | 853625-60-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 4.6 mg/mL (10 mM)), Heating is recommended.
DMSO: 46.1 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.1715 mL | 10.8575 mL | 21.7151 mL | 54.2876 mL |
5 mM | 0.4343 mL | 2.1715 mL | 4.343 mL | 10.8575 mL | |
10 mM | 0.2172 mL | 1.0858 mL | 2.1715 mL | 5.4288 mL | |
DMSO | 20 mM | 0.1086 mL | 0.5429 mL | 1.0858 mL | 2.7144 mL |
50 mM | 0.0434 mL | 0.2172 mL | 0.4343 mL | 1.0858 mL | |
100 mM | 0.0217 mL | 0.1086 mL | 0.2172 mL | 0.5429 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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