Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PF-2771 是一种有效的,选择性的着丝粒蛋白 E (CENP-E) 抑制剂,IC50值为 16.1 nM。PF-2771具有抗肿瘤活性。
产品描述 | PF-2771 is a potent and selective centromere protein E (CENP-E) inhibitor. PF-2771 inhibits CENP-E motor activity with an IC 50 of 16.1 nM. PF-2771 is used as an anticancer agent. |
靶点活性 | CENP-E:16.1 nM |
体外活性 | PF-2771 is a potent and selective CENP-E inhibitor, inhibiting CENP-E motor activity with an IC 50 of 16.1 nM. PF-2771 shows no inhibitory effect on the ATPase activities of highly related kinesins (0% inhibition of Eg5/KSP, chromokinesin, and MCAK at both 1 or 10 μM PF-2771). PF-2771 exhibits inactive activity against 74 protein kinases (all < 23% inhibition with 1 μM PF-2771, < 40% with 10 μM PF-2771). PF-2771 is cytotoxic to the basal-like breast cancer tumor cell survival, with EC 50 s of < 0.1 μM, and with no cytotoxicity on the normal and premalignant cell lines (EC 50 > 5 μM). PF-2771 (100 nM) causes a chromosomal congression defect in MDA-MB-468 cells [1]. |
体内活性 | PF-2771 (100 mg/kg, every day i.p.) potently inhibits CENP-E motor function, and leads to tumor regression in SCID mice bearing AA1077 mammary tumors [1]. |
分子量 | 554.08 |
分子式 | C29H36ClN5O4 |
CAS No. | 2070009-55-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 32 mg/mL (57.75 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.8048 mL | 9.024 mL | 18.0479 mL | 45.1198 mL |
5 mM | 0.361 mL | 1.8048 mL | 3.6096 mL | 9.024 mL | |
10 mM | 0.1805 mL | 0.9024 mL | 1.8048 mL | 4.512 mL | |
20 mM | 0.0902 mL | 0.4512 mL | 0.9024 mL | 2.256 mL | |
50 mM | 0.0361 mL | 0.1805 mL | 0.361 mL | 0.9024 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PF-2771 2070009-55-9 Cytoskeletal Signaling Kinesin PF 2771 PF2771 Inhibitor inhibitor inhibit