Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NVP-TAE 684 (TAE684) 是一种高效特异性 ALK 抑制剂,阻止 ALCL 衍生的 ALK 依赖性细胞株的生长,IC50值为 3 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 367 | 现货 | ||
5 mg | ¥ 625 | 现货 | ||
10 mg | ¥ 961 | 现货 | ||
50 mg | ¥ 2,641 | 现货 | ||
100 mg | ¥ 3,590 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 838 | 现货 |
产品描述 | NVP-TAE 684 (TAE684) is a excellently effective and specific ALK inhibitor(IC50=3 nM). |
靶点活性 | ALK:3 nM |
激酶实验 | In vitro Enzyme Assays.: All in vitro enzyme assays are done at Upstate Biotechnology with the exception of InsR and IGF-1R. To determine the IC50 of TAE684 against InsR and IGF-1R a homogeneous time-resolved fluorescence assay is performed. ATP (10 mM) and 20 mg/ml biotinylated PolyEY (Glu, Tyr 4:1) are combined with 50 nL of serial dilutions of TAE684 (10-500 nM) and 4 ng of InsR enzyme in the presence of the kinase reaction buffer (20 mM Tris譎Cl, pH 7.5/10 mM MgCl2/3 mM MnCl2/1 mM DTT/10 mM NaVO4/0.1 mg/ml of BSA). Assays are incubated for 1 hour at ambient temperature. Reactions are terminated by adding 10 mL of the detection solution containing 50 mM EDTA, 500 mM KF, 0.5 mg/ml of BSA, 5 mg/mL Eu3+ cryptate-labeled anti-phosphotyrosine antibody Mab PT66-K, and 5 mg/mL Streptavidin-XLent. The reaction is incubated for half an hour, and fluorescence signals are read on Analyst GT. |
细胞实验 | Cells are seeded in 384-well plates (2.5×104 cells per well) and incubated with serial dilutions of TAE684 or DMSO for 2–3 days. Luciferase expression is used as a measure of cell proliferation/survival and is evaluated with the Bright-Glo Luciferase Assay System. IC50 values are generated by using XLFit software.(Only for Reference) |
别名 | TAE684, 5-氯-N2-[2-甲氧基-4-[4-(4-甲基-1-哌嗪基)-1-哌啶基]苯基]-N4-[2-[(1-甲基乙基)磺酰基]苯基]-2,4-嘧啶二胺 |
分子量 | 614.2 |
分子式 | C30H40ClN7O3S |
CAS No. | 761439-42-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 6.14 mg/mL (10 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.6281 mL | 8.1407 mL | 16.2813 mL | 40.7034 mL |
5 mM | 0.3256 mL | 1.6281 mL | 3.2563 mL | 8.1407 mL | |
10 mM | 0.1628 mL | 0.8141 mL | 1.6281 mL | 4.0703 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NVP-TAE 684 761439-42-3 Angiogenesis Apoptosis Tyrosine Kinase/Adaptors ALK TAE684 Anaplastic lymphoma kinase Anaplastic lymphoma kinase (ALK) NVPTAE 684 inhibit TAE-684 ALK tyrosine kinase receptor NVP-TAE-684 Inhibitor TAE 684 NVP-TAE684 CD246 5-氯-N2-[2-甲氧基-4-[4-(4-甲基-1-哌嗪基)-1-哌啶基]苯基]-N4-[2-[(1-甲基乙基)磺酰基]苯基]-2,4-嘧啶二胺 Cluster of differentiation 246 NVP TAE 684 inhibitor