Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NF-κB-IN-4 (化合物 17) 是有效的NF-κB 通路抑制剂,可透过血脑屏障(BBB)。NF-κB-IN-4 具有潜在的抗神经炎症活性,毒性低,可用于研究神经炎症相关疾病。NF-κB-IN-4 可阻断 IκBα 的活化及磷酸化,降低 NLRP3 的表达,从而抑制NF-κB 的激活。
产品描述 | NF-κB-IN-4 (compound 17) is a potent inhibitor of NF-κB pathway which can cross blood brain barrier (BBB). NF-κB-IN-4 shows potential anti-neuroinflammatory activity with low toxicity which can be used for the research of neuroinflammation related diseases. NF-κB-IN-4 can block the IκBα activation and phosphorylation, reduce NLRP3 expression, therefore inhibiting NF-κB activation [1]. |
体外活性 | NF-κB-IN-4 (compound 17) (0-10 μM, 24 h) shows high anti-inflammatory activity [1]. NF-κB-IN-4 (0-5 μM, 24 h) significantly decreases the LPS-induced p-IκBα expression levels, significantly inhibits the phosphorylation of IκBα and the expression of three proteins (NLRP3, ASC and caspase-1) in BV2 cells [1]. NF-κB-IN-4 (0-5 μM, 24 h) induces LPS-induced apoptosis in BV2 cells in a dose-dependent manner [1]. Cell Cytotoxicity Assay Cell Line: BV2 microglia cells [1] Concentration: 0, 2.5, 5, 10 μM Incubation Time: 24 h Result: Showed high anti-inflammatory activity, with the survival rate of BV2 microglia cells of 97.4%, inhibition rates against TNF-α and IL-6 release of 60.8% and 80.2%, respectively. Western Blot Analysis Cell Line: BV2 cells [1] Concentration: 0, 1.25, 2.5, 5 μM Incubation Time: 24 h Result: Significantly decreased the LPS-induced p-IκBα expression levels, significantly inhibited the phosphorylation of IκBα and the expression of three proteins (NLRP3, ASC and caspase-1) in BV2 cells. Apoptosis Analysis Cell Line: BV2 cells [1] Concentration: 0, 2.5, 5 μM Incubation Time: 24 h Result: Induced LPS-induced apoptosis in BV2 cells in a dose-dependent manner, with the apoptosis rates of 30.7% (2.5 μM) and 13.0% (5 μM), respectively. |
分子量 | 322.34 |
分子式 | C18H15FN4O |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NF-κB-IN-4 Inhibitor inhibitor inhibit