Powder: -20°C for 3 years | In solvent: -80°C for 1 year
N1-Methylpseudouridine (1-Methylpseudouridine) 是一种甲基假尿苷,翻译性能比 5 mC 和 5 mC/N1-Methylpseudouridine 高。通过提高核糖体密度,mRNA 中的 N1-Methylpseudouridine 以 eIF2α 依赖性和独立机制增强翻译。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 255 | 现货 | ||
10 mg | ¥ 413 | 现货 | ||
25 mg | ¥ 828 | 现货 | ||
50 mg | ¥ 1,330 | 现货 | ||
100 mg | ¥ 1,970 | 现货 | ||
500 mg | ¥ 4,850 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 272 | 现货 |
产品描述 | N1-Methylpseudouridine (1-Methylpseudouridine) is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA. |
体外活性 | In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA .?Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation.?In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate[1]. |
体内活性 | mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection[2]. |
别名 | N1-甲基-假尿苷, 1-Methylpseudouridine |
分子量 | 258.23 |
分子式 | C10H14N2O6 |
CAS No. | 13860-38-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 40 mg/mL (154.90 mM)
DMSO: 100 mg/mL (387.25 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 3.8725 mL | 19.3626 mL | 38.7252 mL | 96.8129 mL |
5 mM | 0.7745 mL | 3.8725 mL | 7.745 mL | 19.3626 mL | |
10 mM | 0.3873 mL | 1.9363 mL | 3.8725 mL | 9.6813 mL | |
20 mM | 0.1936 mL | 0.9681 mL | 1.9363 mL | 4.8406 mL | |
50 mM | 0.0775 mL | 0.3873 mL | 0.7745 mL | 1.9363 mL | |
100 mM | 0.0387 mL | 0.1936 mL | 0.3873 mL | 0.9681 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
N1-Methylpseudouridine 13860-38-3 Others GFP Inhibitor mRNA inhibit DNA/RNA Synthesis eIF2α Nucleoside Antimetabolite/Analog phosphorylation N1-甲基-假尿苷 protein expression N1 Methylpseudouridine N-1-Methylpseudouridine cells HEK293T 1-Methylpseudouridine polysomes ribosome N1Methylpseudouridine inhibitor