Powder: -20°C for 3 years | In solvent: -80°C for 1 year
mTOR inhibitor-8 是一种有效的 mTOR 抑制剂和自噬诱导剂,具有抗病毒和抗肿瘤活性。mTOR inhibitor-8 抑制A549细胞的生长,可用于研究非小细胞肺癌。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 1,470 | 现货 | ||
5 mg | ¥ 2,480 | 现货 | ||
10 mg | ¥ 3,980 | 现货 | ||
25 mg | ¥ 6,670 | 现货 | ||
50 mg | ¥ 8,890 | 现货 | ||
100 mg | ¥ 12,200 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 2,730 | 现货 |
产品描述 | mTOR inhibitor-8 is a potent mTOR inhibitor and autophagy inducer with antiviral and antitumor activity. mTOR inhibitor-8 inhibits the growth of A549 cells, which can be used to study non-small cell lung cancer. |
体外活性 |
mTOR inhibitor-8 (Compound 5e; 0.1-10 μM) suppresses the growth of A549 cells in a dose-dependent manner after 24 and 48 hours of treatment[1]. mTOR inhibitor-8 induces autophagy in an mTOR-dependent manner. At a concentration of 10 μM, mTOR inhibitor-8 induces autophagy in a time-dependent manner (3-24 hours), leading to enhanced levels of LC3B-II[1].At a concentration of 10 μM for 3-24 hours, mTOR inhibitor-8 reduces the phosphorylation of RPS6KB1 (ribosomal protein S6 kinase) and EIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1), two essential substrates of mTOR[1]. |
体内活性 | In vivo, mTOR inhibitor-8 effectively inhibits tumor growth at concentrations of 25 and 50 μM for a duration of 6 days, without adversely affecting angiogenesis in the normal chick chorioallantoic membrane [1]. |
别名 | mTOR-IN-8 |
分子量 | 446.95 |
分子式 | C24H19ClN4OS |
CAS No. | 2489196-70-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 20 mg/mL (44.75 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.2374 mL | 11.1869 mL | 22.3739 mL | 55.9347 mL |
5 mM | 0.4475 mL | 2.2374 mL | 4.4748 mL | 11.1869 mL | |
10 mM | 0.2237 mL | 1.1187 mL | 2.2374 mL | 5.5935 mL | |
20 mM | 0.1119 mL | 0.5593 mL | 1.1187 mL | 2.7967 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
mTOR inhibitor-8 2489196-70-3 Autophagy PI3K/Akt/mTOR signaling mTOR mTOR inhibitor 8 mTOR inhibitor8 mTOR-IN-8 Inhibitor inhibitor inhibit