keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Methylene Blue (Tetramethylthionine chloride) 是鸟苷酸环化酶,是单胺氧化酶 A 和 NO 合酶抑制剂。它有抗伤害感受、抗疟疾、抗抑郁和抗焦虑作用,可研究高铁血红蛋白血症、神经退行性疾病和异环磷酰胺引起的脑病。它是血管加压剂,用作色素内窥镜检查中的染料。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 239 | 现货 | ||
100 mg | ¥ 418 | 现货 | ||
500 mg | ¥ 713 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 164 | 现货 |
产品描述 | Methylene Blue (Tetramethylthionine chloride) (IC50 of 1.9 μM) inhibits tau filament formation. And it inhibits soluble guanylyl cyclase. It is also used as a dye in chromoendoscopy. |
靶点活性 | Tau aggregation:1.9 μM, GC: |
体外活性 | MB could inactivate nitric oxide directly by generating superoxide anions, it may inhibit the action of nitric oxide synthase itself. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, there are MB directly activating calcium-dependent potassium channels and enhancing the release of noradrenaline from intracellular stores. |
体内活性 | Methylene Blue decreases C-GMP and vascular smooth muscle relaxation by inhibiting guanylate cyclase. Pharmacokinetic studies in rodents showed that MB injects by intravascular resulting in a rapid and extensive accumulation of this drug in the central nervous system (CNS). When MB is injected intravenously, it selectively gathers in parathyroid glands, therefore is easy for the identification of structures during surgery. |
激酶实验 | Isolated Integrin Binding Assays.:Purified integrin (1 μg/mL; 4℃) is used to coat 96-well microtitre plates, which are then blocked with bovine serum albumin (BSA) (3% in 1 mM CaCl2, 1 mM MgCl2,10 pM MnCl2, 100 mM NaC1,50 mM Tris-hydroxymethyl-aminomethane; pH 7.4), and incubated (3 h at 30 ℃) with biotinylated ligands (1 pg/mL in binding buffer: 0.1% BSA, 1 mM CaCl2,1 mM MgCl2, 10 μM MnCl2, 100 mM NaCl, 50 mM Tris-hydroxymethyl-aminomethane; pH 7.4) in the presence or absence of serially diluted peptides. After washing (3×5 min with binding buffer), the bound biotinylated ligand is detected with alkaline-phosphatase conjugated goat anti-biotin antibodies (1 μg/mL; 1 h, 37℃), using p-nitrophenyl phosphate as chromogen. Cyclo (-RGDfK) binding in the absence of competitor is defined as 100% signal; binding to blocked wells in the absence of integrin is defined as 0%. Concentrations of Cyclo (-RGDfK) required for 50% inhibition of signal (IC50 values) are estimated graphically. |
细胞实验 | Immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are exposed at the sixth day in vitro to MB (10 μM) for 2h. Assess the survival and dendritic arbor architecture of these cells 48h after this treatment paradigm. |
动物实验 | MB (5 or 50 mg/kg) diluted in normal saline were injected intraarterially into Sprague- Dawley male rats over the 30s. |
别名 | 亚甲蓝, methylthioninium chloride, CI-52015, Tetramethylthionine chloride, 亚甲兰, Basic Blue 9 |
分子量 | 319.85 |
分子式 | C16H18ClN3S |
CAS No. | 61-73-4 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 3.2 mg/mL (10 mM)
DMSO: 1mg/ml, Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 3.1265 mL | 15.6323 mL | 31.2647 mL | 78.1616 mL |
5 mM | 0.6253 mL | 3.1265 mL | 6.2529 mL | 15.6323 mL | |
10 mM | 0.3126 mL | 1.5632 mL | 3.1265 mL | 7.8162 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Methylene Blue 61-73-4 Cytoskeletal Signaling GPCR/G Protein Immunology/Inflammation Microbiology/Virology Neuroscience NO Synthase Microtubule Associated Guanylate cyclase Parasite Monoamine Oxidase antimalarial anxiolytic 亚甲蓝 inhibit Methylthioninium methemoglobinemias cGMP CI 52015 Nitric oxide synthases methylthioninium chloride NOS Tetramethylthionine Chloride Inhibitor antinociception CI-52015 CI52015 Tetramethylthionine chloride antidepressant dye methylthioninium Chloride MAO vasopressor Basic Blue-9 Guanylate Cyclase Basic Blue9 亚甲兰 Basic Blue 9 inhibitor