Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Levoleucovorin Calcium (CL307782) 是一种亚叶酸的钙盐,在癌症化疗中用作辅助剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 233 | 现货 | ||
25 mg | ¥ 372 | 现货 | ||
50 mg | ¥ 538 | 现货 | ||
100 mg | ¥ 788 | 现货 | ||
500 mg | ¥ 1,730 | 现货 | ||
1 g | ¥ 2,570 | 现货 | ||
2 g | ¥ 3,390 | 现货 |
产品描述 | Levoleucovorin Calcium (CL307782), a calcium salt of the folinic acid, is used in cancer chemotherapy as an adjuvant. |
激酶实验 | Chk1 Assay: For the Chk1 assay, the kinase domain is expressed in Sf9 insect cells, and a biotinylated cdc25c peptide containing the consensus Chk1/Chk2 phosphorylation site (*)(biotin-[AHX]SGSGS*GLYRSPSMP-ENLNRPR[CONH2]) is used as the substrate. A dilution series of CHIR-124 is mixed with a kinase reaction buffer containing a final concentration of 30 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM DTT, 4 mM EDTA, 25 mMβ-glycerophosphate, 5 mM MnCl2, 0.01% bovine serum albumin, 1.35 nM CHK1 kinase domain, 0.5 μM peptide substrate, and 1 AM unlabeled ATP, plus 5 nM 33Pγ-labeled ATP (specific activity = 2,000 Ci/mmol). Reactions and detection of the phosphate transfer are carried out by a radioactive method. Reactions are incubated at room temperature for 1 to 4 hours and the phosphorylated peptide captured on streptavidin-coated microtiter plates containing stop reaction buffer (25 mM EDTA [ethylenediaminetetraacetic acid], 50 mMHEPES, pH 7.5). Phosphorylated peptide is measured with the DELFIA TRF system using a Europium-labeled anti-phosphotyrosine antibody PT66. The concentration of CHIR-124 for IC50 is calculated using nonlinear regression with XL-Fit data analysis software. |
别名 | 左亚叶酸钙, Calcium Levofolinate, CL307782 |
分子量 | 511.5 |
分子式 | C20H21N7O7·Ca |
CAS No. | 80433-71-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Insoluble, The compound is unstable in solution, please use soon.
H2O: 10 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 1.955 mL | 9.7752 mL | 19.5503 mL | 48.8759 mL |
5 mM | 0.391 mL | 1.955 mL | 3.9101 mL | 9.7752 mL | |
10 mM | 0.1955 mL | 0.9775 mL | 1.955 mL | 4.8876 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Levoleucovorin Calcium 80433-71-2 Cell Cycle/Checkpoint Others Antifolate CL-307782 左亚叶酸钙 inhibit anticancer Calcium Levofolinate CL 307782 5-Fluorouracil antineoplastic Levoleucovorin Inhibitor CL307782 inhibitor