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JC-1

产品编号 T15609 CAS 3520-43-2

别名: CBIC2

JC-1 (CBIC2) 是一种荧光亲脂性羰花青染料，用于测量线粒体膜电位。线粒体膜电位较高时，它在基质中汇聚形成聚合物，可以产生红色荧光(λex=585 nm，λem=590 nm)。线粒体膜电位较低时，它不能聚集在线粒体基质中，以单体形式存在产生绿色荧光(λex=510 nm，λem=527 nm)。

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JC-1, CAS 3520-43-2

规格	价格/CNY	货期
1 mg	￥ 573	现货
2 mg	￥ 949	现货
5 mg	￥ 1,890	现货
10 mg	￥ 3,170	现货
25 mg	￥ 5,190	现货
50 mg	￥ 7,360	现货
100 mg	￥ 9,920	现货

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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述

JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye. It has been used to measure mitochondrial membrane potential. JC 1 can be used in a probe for measuring mitochondrial membrane potential by flow cytometry.

体外活性

JC-1 fluorescence is usually excited by the 488nm laser wavelength common in flow cytometers[1]. JC-1 (2.5μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3].

别名	CBIC2
分子量	652.23
分子式	C25H27Cl4IN4
CAS No.	3520-43-2

存储

keep away from direct sunlight　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 14 mg/mL(21.46 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5332 mL	7.666 mL	15.332 mL	38.33 mL
	5 mM	0.3066 mL	1.5332 mL	3.0664 mL	7.666 mL
	10 mM	0.1533 mL	0.7666 mL	1.5332 mL	3.833 mL
	20 mM	0.0767 mL	0.3833 mL	0.7666 mL	1.9165 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

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参考文献

1. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430. 2. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462(5): 693-708. 3. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323. 4. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82.

文献引用

1. Wang X, Shi W, Wang K, et al.Rationally Designed Nonapeptides with Great Skin Photoprotective Effect through Producing Type 1 Collagen and Blocking the mTOR Pathway.Journal of Medicinal Chemistry.2023

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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