DPTIP is an effective inhibitor of neutral sphingomyelinase 2 with an IC50 value of 30 nM.
DPTIP (30 μM) decreases exosome release by 50% in astrocytes. DPTIP(0.03-30 μM) dose-dependently blocks EV secretion.
In mice, DPTIP potently (10 mg/kg; i.p.) inhibits IL-1β-induced astrocyte-derived EV release. Brain concentrations of DPTIP are above its IC50 for neutral sphingomyelinase 2 inhibition for at least 4 h after the administration of DPTIP.