Powder: -20°C for 3 years | In solvent: -80°C for 1 year
D-Lin-MC3-DMA 是一种递送 siRNA 载体,是一种可离子化的氨基脂质类化合物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 436 | 现货 | ||
2 mg | ¥ 636 | 现货 | ||
5 mg | ¥ 997 | 现货 | ||
10 mg | ¥ 1,530 | 现货 | ||
25 mg | ¥ 2,960 | 现货 | ||
50 mg | ¥ 4,370 | 现货 | ||
100 mg | ¥ 6,220 | 现货 | ||
500 mg | ¥ 12,700 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,410 | 现货 |
产品描述 | D-Lin-MC3-DMA, an ionizable cationic lipid, is a potent siRNA delivery vehicle. |
体外活性 | 将DSPC替换为不饱和PCs,并将DLin-MC3-DMA替换为相关脂质DLin-KC2-DMA,可显著提高HeLa细胞体外转染试剂的效能。 |
细胞实验 | White leghorn chicken embryos at stage 24 (30) were removed and dissected in Hanks buffered saline solution (HBSS).?Forelimbs were pooled and treated for 1 h in dispase to remove epithelial ectoderm.?Limb mesenchyme was dissociated, and resuspended in DMEM with 5% FBS and 1% penicillin/streptomycin.?Cells were plated onto 96-well plates at a density of 2x10^5 or 2x10^7 cells/ml and treated with 0-40 μg/ml LNP-pDNA.?Cultures were incubated overnight at 37℃ and resuspended in PBS buffer for expression analysis using a BD LSRII flow cytometer.?Cell survival was assessed in low-density cultures after 24 h of treatment, by counting the adherent cells remaining after PBS washes.?Viability was determined based on average counts of all adherent cells within a single field of view (100x) normalized to cell counts of untreated cultures. |
分子量 | 642.09 |
分子式 | C43H79NO2 |
CAS No. | 1224606-06-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 16.67 mg/mL (25.96 mM)
DMSO: 43.33 mg/mL (67.48 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 1.5574 mL | 7.7871 mL | 15.5741 mL | 38.9354 mL |
5 mM | 0.3115 mL | 1.5574 mL | 3.1148 mL | 7.7871 mL | |
10 mM | 0.1557 mL | 0.7787 mL | 1.5574 mL | 3.8935 mL | |
20 mM | 0.0779 mL | 0.3894 mL | 0.7787 mL | 1.9468 mL | |
DMSO | 50 mM | 0.0311 mL | 0.1557 mL | 0.3115 mL | 0.7787 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
D-Lin-MC3-DMA 1224606-06-7 Others Inhibitor D-Lin-MC-3-DMA D Lin MC3 DMA inhibit DLinMC3DMA inhibitor