Powder: -20°C for 3 years | In solvent: -80°C for 1 year
D-(+)-Melezitose hydrate (melicitose) 是由许多以植物汁液为食的昆虫通过酶反应产生的一种非还原性三糖,能鉴定吲哚阳性和吲哚阴性克雷伯菌属的临床分离株。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 150 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 130 | 现货 |
产品描述 | D-(+)-Melezitose hydrate (melicitose) is a nonreducing trisaccharide sugar that is produced by many plant sap-eating insects, including aphids such as Cinara pilicornis by an enzyme reaction. |
激酶实验 | FlashPlate assay (96-well screening assay): To columns 1 through 10, 1 μL of Olaparib (in DMSO) is added, and 1 μL DMSO only is added to the positive (POS) and negative (NEG) control wells (columns 11 and 12, respectively) of a pretreated FlashPlate. PARP-1 is diluted 1:40 in buffer (buffer B: 10% glycerol (v/v), 25 mM HEPES, 12.5 mM MgCl2,50 mM KCl, 1 mM DTT, 0.01% NP-40 (v/v), pH 7.6) and 40 μL added to all 96 wells (final PARP-1 concentration in the assay is ~1 ng/μL). The plate is sealed and shaken at RT for 15 min. Following this, 10 μL of positive reaction mix (0.2 ng/μL of double-stranded oligonucleotide [M3/M4] DNA per well, 5 μM of NAD+ final assay concentration, and 0.075 μCi 3H-NAD+ per well) is added to the appropriate wells (columns 1-11). The negative reaction mix, lacking the DNA oligonucleotide, is added to column 12 (with the mean negative control value used as the background). The plate is resealed and shaken for a further 60 min at RT to allow the reaction to continue. Then, 50 μL of ice-cold acetic acid (30%) is added to each well to stop the reaction, and the plate is sealed and shaken for a further 60 min at RT. Tritiated signal bound to the FlashPlate is then determined in counts per minute (CPM) using the TopCount plate reader. |
别名 | D-Melezitose, melicitose, Melezitose, 松三糖 |
分子量 | 504.45 |
分子式 | C18H34O17 |
CAS No. | 207511-10-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (114.84 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.9824 mL | 9.9118 mL | 19.8236 mL | 49.5589 mL |
5 mM | 0.3965 mL | 1.9824 mL | 3.9647 mL | 9.9118 mL | |
10 mM | 0.1982 mL | 0.9912 mL | 1.9824 mL | 4.9559 mL | |
20 mM | 0.0991 mL | 0.4956 mL | 0.9912 mL | 2.4779 mL | |
50 mM | 0.0396 mL | 0.1982 mL | 0.3965 mL | 0.9912 mL | |
100 mM | 0.0198 mL | 0.0991 mL | 0.1982 mL | 0.4956 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
D-(+)-Melezitose hydrate 207511-10-2 Microbiology/Virology Others Antibacterial Inhibitor Bacterial D-Melezitose (+)-Melezitose melicitose D(+)Melezitose hydrate D (+) Melezitose hydrate Melezitose inhibit D-Melezitose Hydrate 松三糖 inhibitor