Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Coenzyme FO, a deazaflavin chromophore, plays a crucial role as a hydride acceptor/donor in the central methanogenic pathway [1][2].
产品描述 | Coenzyme FO, a deazaflavin chromophore, plays a crucial role as a hydride acceptor/donor in the central methanogenic pathway [1][2]. |
体外活性 | The formation of Coenzyme FO is mediated by two separate radical SAM active sites, one each in the CofG and CofH enzymes or both in the FbiC enzyme. These two radical SAM domains constitute the functional domains of Fo synthase. F420 is biosynthesized in Methanocaldococcus jannaschii by the action of eight enzymes with the formation of the deazaflavin chromophore (Fo) as the remaining unsolved step[1].Coenzyme F420 is the central low-redox-potential electron carrier in methanogenic metabolism. Coenzyme F420 is reduced under hydrogen by the action of F420-dependent hydrogenase[3].Coenzyme F420 acts as a hydride transfer coenzyme for an F420-specific glucose-6-phosphate dehydrogenase (Fgd) in mycobacteria. Coenzyme F420 is found in all methanogenic and certain nonmethanogenic archaea, where it participates in energy metabolism, NADP reduction, oxygen detoxification, and sulfite reduction. By converting NO2 back to NO with F420H2, M. tuberculosis could decrease the effectiveness of antibacterial action of macrophages[4]. |
分子量 | 363.326 |
分子式 | C16H17N3O7 |
CAS No. | 37333-48-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5 mg/mL (13.76 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.7523 mL | 13.7617 mL | 27.5235 mL | 68.8087 mL |
5 mM | 0.5505 mL | 2.7523 mL | 5.5047 mL | 13.7617 mL | |
10 mM | 0.2752 mL | 1.3762 mL | 2.7523 mL | 6.8809 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Coenzyme FO 37333-48-5 Inhibitor inhibitor inhibit