Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CAY10603 (BML-281) 是一种选择性 HDAC6抑制剂,IC50值为 2 pM。它也可抑制 HDAC1、HDAC2、HDAC3、HDAC8 和 HDAC10,IC50值分别为 271、252、0.42、6851和90.7 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 362 | 现货 | ||
2 mg | ¥ 523 | 现货 | ||
5 mg | ¥ 887 | 现货 | ||
10 mg | ¥ 1,470 | 现货 | ||
25 mg | ¥ 2,890 | 现货 | ||
50 mg | ¥ 4,280 | 现货 | ||
100 mg | ¥ 5,970 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 889 | 现货 |
产品描述 | CAY10603 (BML-281) is a potent and selective inhibitor of HDAC6. |
靶点活性 | HDAC6:2 pM |
体内活性 | CAY10603通过抑制HDAC6,表现出对胰腺癌细胞系有效的抗增殖活性,IC50 <1 μM。 |
激酶实验 | HDAC Inhibition Assays: Purified HDACs are incubated with 1 mM carboxyfluorescein (FAM)-labeled acetylated peptide substrate and test compound for 17 h at 25 °C in HDAC assay buffer containing 100 mM HEPES (pH 7.5), 25 mM KCl, 0.1% BSA, and 0.01% Triton X-100. Reactions are terminated by the addition of buffer containing 0.078% SDS for a final SDS concentration of 0.05%. Substrate and product are separated electrophoretically using a Caliper LabChip 3000 system with blue laser excitation and green fluorescence detection (CCD2). The fluorescence intensity in the substrate and product peaks is determined using the Well Analyzer software on the Caliper system. The reactions are performed in duplicate for each sample. IC50 values are automatically calculated using the IDBS XLFit version 4.2.1 plug-in for Microsoft Excel and the XLFit 4-Parameter Logistic Model: ((A+((B_A)/1+((C/x)D)))), in which x is compound concentration, A and B are respectively the estimated minimum and maximum of percent inhibition, C is the inflection point, and D is the Hill slope of the sigmoidal curve. The standard errors of the IC50 values are automatically calculated using the IDBS XLFit version 4.2.1 plug-in for Microsoft Excel and the formula xf4_FitResultStdError. |
细胞实验 | The pancreatic cancer cell lines BxPc-3, HupT3, Mia Paca-2, Panc 04.03, and SU 86.86 are grown in medium (DMEM or RPMI) containing 10% fetal calf serum and l-glutamine. Pancreatic cancer cells are plated out in duplicate into 6 wells of a 96-well microtiter plate. Four hours post plating, individual wells are treated with diluent (DMSO) or varying concentrations of SAHA or the indicated HDACIs from a concentration of 1 nM to 50 mM. Cytotoxicity is measured at time "0", and 72 h post treatment using the colorimetric MTT assay according to the manufacturer's suggestions. The IC50 values are calculated using XLfit.(Only for Reference) |
别名 | HDAC6 Inhibitor, BML-281 |
分子量 | 446.5 |
分子式 | C22H30N4O6 |
CAS No. | 1045792-66-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 5 mg/mL (11.19 mM), Heating is recommended.
DMSO: 82 mg/mL (183.7 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.2396 mL | 11.1982 mL | 22.3964 mL | 55.991 mL |
5 mM | 0.4479 mL | 2.2396 mL | 4.4793 mL | 11.1982 mL | |
10 mM | 0.224 mL | 1.1198 mL | 2.2396 mL | 5.5991 mL | |
DMSO | 20 mM | 0.112 mL | 0.5599 mL | 1.1198 mL | 2.7996 mL |
50 mM | 0.0448 mL | 0.224 mL | 0.4479 mL | 1.1198 mL | |
100 mM | 0.0224 mL | 0.112 mL | 0.224 mL | 0.5599 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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