keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
BSJ-03-123是一种有效的 CDK6 选择性小分子降解剂,是由 Cereblon 配体和 CDK 配体相连的 PROTAC。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 779 | 现货 | ||
2 mg | ¥ 1,160 | 现货 | ||
5 mg | ¥ 1,980 | 现货 | ||
10 mg | ¥ 2,830 | 现货 | ||
25 mg | ¥ 4,530 | 现货 | ||
50 mg | ¥ 6,110 | 现货 | ||
100 mg | ¥ 8,420 | 现货 | ||
500 mg | ¥ 16,800 | 现货 |
产品描述 | BSJ-03-123 is a potent, CDK6-selective small-molecule degrader. |
体外活性 | BSJ-03-123 induced rapid, dose-dependent ternary complex formation with CDK6 and CRBN, but not with CDK4 and CRBN. Ternary complex formation was prevented by blocking binding sites on CDK6 or CRBN via pretreatment with palbo or lenalidomide. |
细胞实验 | 4x10^6 MV4-11 CRBN-/- cells were treated for 3 h with 20 μM palbociclib, BSJ-03-123 or vehicle. 1x10^6 cells were spun down at 500 x g for 5 min, and the supernatant removed. Pellets were incubated at 46, 49, 52 or 55°C for 3 min followed by 3 min incubation at room temperature. 30 μl of lysis buffer (20 mM Tris-HCl pH 8.0, 120 mM NaCl, 0.5% NP-40, protease inhibitors) were added and cells lysed by 3 rounds of snap freezing and thawing. Denatured proteins were removed by 20 min centrifugation at 14.000 x g at 4°C and supernatants analyzed by Western Blotting. |
分子量 | 937.01 |
分子式 | C47H56N10O11 |
CAS No. | 2361493-16-3 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 115 mg/mL (122.73 mM)
H2O: Insoluble
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.0672 mL | 5.3361 mL | 10.6722 mL | 26.6806 mL |
5 mM | 0.2134 mL | 1.0672 mL | 2.1344 mL | 5.3361 mL | |
10 mM | 0.1067 mL | 0.5336 mL | 1.0672 mL | 2.6681 mL | |
20 mM | 0.0534 mL | 0.2668 mL | 0.5336 mL | 1.334 mL | |
50 mM | 0.0213 mL | 0.1067 mL | 0.2134 mL | 0.5336 mL | |
100 mM | 0.0107 mL | 0.0534 mL | 0.1067 mL | 0.2668 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
BSJ-03-123 2361493-16-3 Cell Cycle/Checkpoint CDK Inhibitor PROTACs Cyclin dependent kinase inhibit BSJ03123 BSJ 03 123 inhibitor