Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Alibendol (cebera) 具有促胆汁分泌及抗痉挛的作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 mg | ¥ 194 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 460 | 现货 |
产品描述 | Alibendol (cebera) is a choleretic, antispasmodic, and cholekinetic. |
激酶实验 | Enzyme Assays: Assays measuring the enzyme-catalyzed phosphorylation of GST-IκBα are performed by adding enzyme (a final concentration of 0.5 μg/mL) at 30 ℃ to solutions of 100 μg/mL GST- IκBα and 5 μM [33P]ATP in 40 mM Tris HCl, pH 7.5, containing 4 mM MgCl2, 34 mM sodium phosphate, 3 mM NaCl, 0.6 mM potassium phosphate, 1 mM KCl, 1 mM dithiothreitol, 3% (w/v) glycerol, and 250 μg/mL bovine serum albumin. The specific activity of [33P]ATP used in the assay is 100 Ci/mmol. After 5 min, the kinase reactions are stopped by the addition of 2× Laemmli sample buffer and heat-treated at 90 ℃ for 1 min. The samples are then loaded on to NuPAGE 10% BisTris gels. After completion of SDS-PAGE, gels are dried on a slab gel dryer. The bands are then detected using a 445Si PhosphorImager, and the radioactivity is quantified using ImageQuant software. Under these conditions, the degree of phosphorylation of GST-IκBα is linear with time and concentration of enzyme. |
别名 | cebera, EB 1856, 阿利苯多 |
分子量 | 251.28 |
分子式 | C13H17NO4 |
CAS No. | 26750-81-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 47 mg/mL (187 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 47 mg/mL (187 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 3.9796 mL | 19.8981 mL | 39.7962 mL | 99.4906 mL |
5 mM | 0.7959 mL | 3.9796 mL | 7.9592 mL | 19.8981 mL | |
10 mM | 0.398 mL | 1.9898 mL | 3.9796 mL | 9.9491 mL | |
20 mM | 0.199 mL | 0.9949 mL | 1.9898 mL | 4.9745 mL | |
50 mM | 0.0796 mL | 0.398 mL | 0.7959 mL | 1.9898 mL | |
100 mM | 0.0398 mL | 0.199 mL | 0.398 mL | 0.9949 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Alibendol 26750-81-2 Others cebera EB-1856 inhibit EB 1856 EB1856 阿利苯多 Inhibitor inhibitor