store at low temperature,store under nitrogen,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) 是荧光过氧化物酶底物,其λex=530 nm,λem=590 nm。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 247 | 现货 | ||
5 mg | ¥ 690 | 现货 | ||
10 mg | ¥ 987 | 现货 | ||
25 mg | ¥ 1,730 | 现货 | ||
50 mg | ¥ 2,580 | 现货 | ||
100 mg | ¥ 3,790 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 636 | 现货 |
产品描述 | ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm). |
体外活性 | To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6/s. The kobs also increase over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second-order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s. |
别名 | 10-乙酰基-3,7-二羟基吩嗪, 10-Acetyl-3,7-dihydroxyphenoxazine |
分子量 | 257.24 |
分子式 | C14H11NO4 |
CAS No. | 119171-73-2 |
store at low temperature,store under nitrogen,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/ml (233.25 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.8874 mL | 19.4371 mL | 38.8742 mL | 97.1855 mL |
5 mM | 0.7775 mL | 3.8874 mL | 7.7748 mL | 19.4371 mL | |
10 mM | 0.3887 mL | 1.9437 mL | 3.8874 mL | 9.7186 mL | |
20 mM | 0.1944 mL | 0.9719 mL | 1.9437 mL | 4.8593 mL | |
50 mM | 0.0777 mL | 0.3887 mL | 0.7775 mL | 1.9437 mL | |
100 mM | 0.0389 mL | 0.1944 mL | 0.3887 mL | 0.9719 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ADHP 119171-73-2 Others 10-乙酰基-3,7-二羟基吩嗪 10-Acetyl-3,7-dihydroxyphenoxazine inhibit Inhibitor inhibitor