Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Zebularine (4-Deoxyuridine) 是一种 DNA 甲基化抑制剂,还抑制胞苷脱氨酶。它通过以共价水合物的形式与活性大小结合,充当胞苷脱氨酶的过渡态类似物抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 226 | 现货 | ||
5 mg | ¥ 359 | 现货 | ||
10 mg | ¥ 578 | 现货 | ||
25 mg | ¥ 1,190 | 现货 | ||
50 mg | ¥ 2,320 | 现货 | ||
100 mg | ¥ 3,460 | 现货 | ||
500 mg | ¥ 7,520 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 398 | 现货 |
产品描述 | Zebularine (4-Deoxyuridine) is a DNA methylation inhibitor. Acts as a transition state analog inhibitor of cytidine deaminase by binding to the active size as covalent hydrates. It also inhibits cytidine deaminase (Ki: 2 μM, in a cell-free assay). |
靶点活性 | Cytidine deaminase:2 μM(Ki) |
体外活性 | Zebularine(1000 mg/kg)对携带肿瘤的小鼠具有轻微细胞毒性,平均最大重量变化为11%(95%可信区间:4%-19%).与对照组相比,高剂量Zebularine(i.p/p.o.)处理可显著减小小鼠的肿瘤体积. |
体内活性 | Zebularine可与细菌甲基转移酶形成紧密共价复合物。作用于N. crassa时,Zebularine使DNA甲基化受到抑制,并使甲基化导致的沉默基因被重新激活。作用于T24膀胱癌细胞时,Zebularine使沉默的p16基因被重新激活,且使其启动子区域去甲基化。Zebularine对T24细胞只有轻微的细胞毒性。与正常成纤维细胞相比,Zebularine会优先掺入癌细胞系的DNA,且更大程度抑制其细胞生长与基因表达。此外,与正常成纤维细胞相比,Zebularine会首先抑制癌细胞的DNA甲基转移酶1,并诱导癌症相关的抗原基因表达。 |
细胞实验 | For methylation analysis, 10T1/2 cells and T24 cells are treated with the various concentrations of zebularine. For 10T1/2 cells, the medium is changed 24 hours after the initial drug treatment, whereas for T24 cells, the medium is changed 24 hours or 48 hours after the initial drug treatment. DNA and RNA are harvested from 10T1/2 cells 72 hours after initial drug treatment and from T24 cells 96 hours after initial drug treatment. The methylation status of the indicated DNA regions is measured in two separate and independent experiments, both of which are done in duplicate[2]. |
别名 | NSC309132, 4-Deoxyuridine |
分子量 | 228.2 |
分子式 | C9H12N2O5 |
CAS No. | 3690-10-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 22.8 mg/mL (100 mM)
H2O: 22.8 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 4.3821 mL | 21.9106 mL | 43.8212 mL | 109.553 mL |
5 mM | 0.8764 mL | 4.3821 mL | 8.7642 mL | 21.9106 mL | |
10 mM | 0.4382 mL | 2.1911 mL | 4.3821 mL | 10.9553 mL | |
20 mM | 0.2191 mL | 1.0955 mL | 2.1911 mL | 5.4777 mL | |
50 mM | 0.0876 mL | 0.4382 mL | 0.8764 mL | 2.1911 mL | |
100 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0955 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Zebularine 3690-10-6 Autophagy Chromatin/Epigenetic DNA Methyltransferase DNMTs Inhibitor NSC309132 DNA MTases NSC-309132 4-Deoxyuridine NSC 309132 inhibit inhibitor