Powder: -20°C for 3 years | In solvent: -80°C for 1 year
YH239-EE 是 YH239 的乙酯,是 p53-MDM2 拮抗剂和凋亡诱导剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 225 | 现货 | ||
5 mg | ¥ 525 | 现货 | ||
10 mg | ¥ 847 | 现货 | ||
25 mg | ¥ 1,730 | 现货 | ||
50 mg | ¥ 3,370 | 现货 | ||
100 mg | ¥ 4,790 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 932 | 现货 |
产品描述 | YH239-EE, the ethyl ester of YH239, is a potent p53-MDM2 antagonist and an apoptosis inducer. |
体外活性 | YH239-EE inhibits the growth of OCI-AML-3 cells with wild type p53 by inhibiting the p53-MDM2 interaction. YH239-EE induces cell cycle arrest and causes potent cell apoptosis via activation of p53 and downstream targets in four AML cells (OCI-AML-3 and MOLM-13 with wt p53, NB4 with p53 mutation, and HL60 with p53 deletion). [1] |
细胞实验 | Cell lines are harvested in log-phase growth and seeded following exposure to p53-MDM2 antagonists. Cell number and viability are determined by staining cells with Trypan blue 0.4% and counted with Countess slides.(Only for Reference) |
分子量 | 504.41 |
分子式 | C25H27Cl2N3O4 |
CAS No. | 1364488-67-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 15 mg/mL (29.7 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 93 mg/mL (184.4 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 1.9825 mL | 9.9126 mL | 19.8251 mL | 49.5629 mL |
5 mM | 0.3965 mL | 1.9825 mL | 3.965 mL | 9.9126 mL | |
10 mM | 0.1983 mL | 0.9913 mL | 1.9825 mL | 4.9563 mL | |
20 mM | 0.0991 mL | 0.4956 mL | 0.9913 mL | 2.4781 mL | |
DMSO | 50 mM | 0.0397 mL | 0.1983 mL | 0.3965 mL | 0.9913 mL |
100 mM | 0.0198 mL | 0.0991 mL | 0.1983 mL | 0.4956 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
YH239-EE 1364488-67-4 Apoptosis Ubiquitination E1/E2/E3 Enzyme E2 conjugating enzyme Ubiquitin activating enzyme YH239 EE Ubiquitin conjugating enzyme E1 activating enzyme E3 ligating enzyme inhibit Inhibitor YH-239-EE YH239EE MDM-2/p53 Ubiquitin ligase inhibitor