Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Y15 (1,2,4,5-Benzenetetramine tetrahydrochlor) 是一种特异性的粘着斑激酶抑制剂,能够降低其自身磷酸化活性,抑制癌细胞的活力,阻断肿瘤生长。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 197 | 现货 | ||
10 mg | ¥ 327 | 现货 | ||
25 mg | ¥ 512 | 现货 | ||
50 mg | ¥ 690 | 现货 | ||
100 mg | ¥ 1,270 | 现货 | ||
200 mg | ¥ 1,880 | 现货 | ||
500 mg | ¥ 3,230 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 229 | 现货 |
产品描述 | Y15 (1,2,4,5-Benzenetetramine tetrahydrochlor) is a potent and specificsmall-molecule FAK scaffolding inhibitor that inhibits its autophosphorylation activity, blocks tumor growth. |
体外活性 | Y15 inhibited viability in ?thyroid cancer cell lines in a dose-dependent manner[1]. |
细胞实验 | Apoptosis assay was done with PE Annexin V Apoptosis Detection Kit (BD) to identify apoptotic nuclei.?One hundred millimeter plates were seeded with TPC1, K1, BCPAP and TT cells at a concentration of 1 × 10^6 cells in normal growth media.?After overnight incubation, plates were treated with one of several doses of Y15 or left untreated.?After another 24 h of incubation, cells were collected with trypsin, and following centrifugation, were washed with 1× PBS.?Again, cells were centrifuged, PBS was removed and cells were resuspended in binding buffer and filtered through BD mesh tubes.?Annexin V and 7-AAD were added to the solution, vortexed and incubated for 15 minutes in the dark.?Binding buffer was added and the cells were analyzed by flow cytometry within 1 hour. |
别名 | 1,2,4,5-苯四胺四盐酸盐, 1,2,4,5-Benzenetetramine tetrahydrochlor, FAK Inhibitor 14 |
分子量 | 284.01 |
分子式 | C6H14Cl4N4 |
CAS No. | 4506-66-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 59 mg/mL (207.74 mM), sonification/heating is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 3.521 mL | 17.605 mL | 35.21 mL | 88.0251 mL |
5 mM | 0.7042 mL | 3.521 mL | 7.042 mL | 17.605 mL | |
10 mM | 0.3521 mL | 1.7605 mL | 3.521 mL | 8.8025 mL | |
20 mM | 0.1761 mL | 0.8803 mL | 1.7605 mL | 4.4013 mL | |
50 mM | 0.0704 mL | 0.3521 mL | 0.7042 mL | 1.7605 mL | |
100 mM | 0.0352 mL | 0.1761 mL | 0.3521 mL | 0.8803 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Y15 4506-66-5 Angiogenesis Cytoskeletal Signaling Tyrosine Kinase/Adaptors FAK PTK2 protein tyrosine kinase 2 FAK Inhibitor-14 FAK Inhibitor14 Y-15 1,2,4,5-苯四胺四盐酸盐 PTK2 Focal adhesion kinase 1,2,4,5-Benzenetetramine tetrahydrochlor Y 15 Inhibitor FAK Inhibitor 14 inhibit FAK inhibitor Y15 inhibitor