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WKYMVM

产品编号 T7484 CAS 187986-17-0

WKYMVM 是一种 N-甲酰肽受体FPR1和FPRL1/2的激动剂，可以激活一些白细胞效应功能（如趋化性、激活补体受体-3、NADPH 氧化酶）。

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WKYMVM, CAS 187986-17-0

规格	价格/CNY		货期	数量
1 mg	￥ 3,530		35日内发货

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WKYMVM 的其他形式现货产品:

WKYMVM 2TFA(187986-17-0(free base))

其他形式的 WKYMVM:

WKYMVM 2TFA(187986-17-0(free base))
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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述	WKYMVM is a N-formyl peptide receptor (FPR1) agonist.
体外活性	Neutrophils are activated by WKYMVM through FPRL1 and that FPRL2 is a chemotactic receptor transducing signals in myeloid cells[1].
体内活性	Using blood samples withdrawn from 28 AL patients and 31 healthy controls, found that, in the absence of exogenous WKYMVm, WKYMVm receptor and bactericidal activity did not differ between patients and controls.?Addition of WKYMVm markedly increased the bactericidal activities of these cells in a dose-dependent manner.?During induction chemotherapy, there were significant increases in bactericidal activity in the presence and absence of 1nM WKYMVm, with higher bactericidal activities at the time of complete remission than at the time of diagnosis or on day 15.?During consolidation chemotherapy, WKYMVm had no effect on bactericidal activities.?Patients showed significant increase in the concentrations of TNF alpha, IL-1b, IL-6 and IL-8, but significant decrease in the concentrations of IL-2, IL-4 and IL-12.?TNF alpha, IL-1b and IL-6 showed significant negative correlations with bactericidal activities of patient neutrophils at time of diagnosis, and IL-4 showed a significant positive correlation with bactericidal activities,these findings indicate that WKYMVm enhances bactericidal activity in patients with AL[2].
细胞实验	Ligand-mediated internalization of FPRL1 and FPRL2 was followed by an indirect immunofluorescence microscopy staining technique.?Cells were seeded on polyornithine-coated coverslips 48 h prior to the experiments.?To stop protein synthesis, cells were incubated in RPMI 1640 medium containing 1% BSA and 100 μg/ml cycloheximide for 3 h and maintained in the same medium throughout the experiment.?To study receptor internalization, RINm5F-FPRL1 cells and RINm5F-FPRL2 cells were treated with either WKYMVM or WKYMVm at 37 °C for various time periods ranging from 0 to 30 min. Cells were finally fixed and permeabilized with acetone at ?20 °C for 30 s[1].

分子量	856.11
分子式	C41H61N9O7S2
CAS No.	187986-17-0

存储

keep away from moisture　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 10 mM

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1681 mL	5.8404 mL	11.6807 mL	29.2019 mL
	5 mM	0.2336 mL	1.1681 mL	2.3361 mL	5.8404 mL
	10 mM	0.1168 mL	0.584 mL	1.1681 mL	2.9202 mL

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参考文献

1. Christophe T , Karlsson A , Dugave C , et al. The Synthetic Peptide Trp-Lys-Tyr-Met-Val-Met-NH2 Specifically Activates Neutrophils through FPRL1/Lipoxin A4 Receptors and Is an Agonist for the Orphan Monocyte-expressed Chemoattractant Receptor FPRL2[J]. Journal of Biological Chemistry, 2001, 276(24):21585-21593. 2. Kim H , Noh E K , Lee E J , et al. Enhanced bactericidal function by WKYMVm in patients with acute leukemia[J]. Leukemia Research, 2008, 32(5):717-725.

剂量换算

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体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

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