首页 工具
登录
购物车
Vinflunine Tartrate

Vinflunine Tartrate

产品编号 T6722   CAS 1201898-17-0

Vinflunine Tartrate 是一种独特的新型长春花生物碱,具有抑制有丝分裂和微管蛋白相互作用活性的特性。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
Vinflunine Tartrate Chemical Structure
Vinflunine Tartrate, CAS 1201898-17-0
规格 价格/CNY 货期 数量
1 mg 待询 待询
2 mg 待询 待询
5 mg 待询 待询
10 mg 待询 待询
25 mg 待询 待询
50 mg 待询 待询
1 mL * 10 mM (in DMSO) 待询 待询
其他形式的 Vinflunine Tartrate:
产品目录号及名称: Vinflunine Tartrate (T6722)
点击图片重新获取验证码
更多批次查询请联系客服
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Vinflunine Tartrat is a vinca alkaloid uniquely fluorinated with the properties of mitotic-arresting and tubulin-interacting activity.
体外活性 The major effects of Vinflunine on dynamic instability are a slowing of the microtubule growth rate, an increase in growth duration, and a reduction in shortening duration. The effects of Vinflunine on the readmilling rate is examined by following [3H]GTP incorporation into MAP-rich microtubules, and the IC50 is 0.42 μM. [1] Vinflunine induced mitotic accumulation with IC50 with 18.8 nM, which decreases the centromere dynamicity by 44% and increases the time centromeres spent ina paused state by 63%. [2] Vinflunine ditartrate exhibits microtubule inhibition (purified tubulin and MTP) and cytotoxicity in L1210 cells with IC50 of (0.49 μM and 3.5 μM) and 97 nM, respectively. [3] Vinflunine induces apoptosis in neuroblastoma SK-N-SH cells through a postmitotic G1 arrest and a mitochondrial pathway in a concentration-dependent manner with an IC50 with 50 nM. sup>[4] Treatment of Vinflunine induces a rapid change in endothelial cell shape: cells retracts and assumes a rounded morphology. Mean IC50 values are 9.9 x 10-5 M x 10-5 M for fibronectin and 5.0x 10-5 M x 10-5 M for type IV collagen. A short 4 hours exposure of endothelial cells to Vinflunine at 10-8
体内活性 Intravenous treatment of mice with Vinflunine, immediately before and 2 day after Matrigel implantation, results in a dose-dependent inhibition of the bFGF-induced angiogenic response, compared with vehicle-treated animals. Inhibition of haemoglobin content is significant at 1.25, 2.5 and 5 mg/kg, with no effect at 0.63 mg/kg (P > 0.05). An ID50 value (dose which inhibits 50% of bFGF-induced neovascularisation) is calculated as 1 mg/kg. Low doses of Vinflunine reduce the number of experimental liver metastases by human LS174T colon cancer cell. A slight overall decrease in liver metastatic foci is already observed at the very low dose of 0.16 mg/kg Vinflunine, although maximal overall inhibition is reached at the maximal tolerated dose (MTD) of 20 mg/kg. [5]
激酶实验 Determination of Microtubule Polymer Mass: Purified tubulin (17 μM) is polymerized into microtubules in the abence or presence of a range of vnflunine concentrations (35 minutes; 37 °C) in 75 mM PIPES, 1.8 mM MgCl2, 1.0 mM EGTA, and 1.5 mM GTP (pH 6.8) using sea urchin (Strongylocentrotus purpuratus) axonemes as seeds for assembly initiation. After incubation, polymerized microtubules are separated from unpolymerized tubulin by centrifugation (150,000 × g; 1 hour; 35 °C). The supernatant is aspirated, the sedimented microtubules are depolymerized in assembly buffer by incubation on ice (2 hours), and the protein content is determined.
细胞实验 Effects of Vinflunine on L1210 cell proliferation are determined using a standard growth inhibition assay. Exponentially growing L1210 cells (1.5 × 105 cells/well) in a 24-well plate are exposed to a range of concentrations of test compounds for 48 hours, prior to determining cell numbers using an electronic particle counter based on linear interpolation between data points.(Only for Reference)
分子量 967.02
分子式 C45H54F2N4O8·xC4H6O6
CAS No. 1201898-17-0

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 45 mg/mL (46.5 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0341 mL 5.1705 mL 10.341 mL 25.8526 mL
5 mM 0.2068 mL 1.0341 mL 2.0682 mL 5.1705 mL
10 mM 0.1034 mL 0.5171 mL 1.0341 mL 2.5853 mL
20 mM 0.0517 mL 0.2585 mL 0.5171 mL 1.2926 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
=
X
X
X
=
X
=
/
g/mol

输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Ngan VK, et al, Cancer Res, 2000, 60(18), 5045-5051. 2. Lobert S, et al, Biochemistry, 2000, 39(39), 12053-12062. 3. Okauneva T, et al, Mol Cancer Ther, 2003, 2(5), 427-436. 4. Pourroy B, et al, Mol Pharmacol, 2004, 66(3), 580-591. 5. Kruczynski A, et al, Eur J Cancer, 2006, 42(16), 2821-2832.
Monomethyl auristatin E Taccalonolide B Estramustine phosphate sodium T-900607 Fosbretabulin Disodium Ixabepilone Estramustine INH1

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Vinflunine Tartrate 1201898-17-0 Cytoskeletal Signaling Microtubule Associated Inhibitor inhibitor inhibit

 

陶术
生物
TargetMol®中国区唯一合作伙伴
点击进入陶术生物官网陶术生物
联系我们
400-820-0310

上海市静安区江场三路238号8楼