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VO-Ohpic trihydrate

VO-Ohpic trihydrate

产品编号 T7012   CAS 476310-60-8
别名: VO-Ohpic

VO-Ohpic trihydrate (VO-Ohpic) 是一种有效的磷酸酶和张力蛋白同源物(PTEN)抑制剂,IC50 为 35 nM。

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VO-Ohpic trihydrate Chemical Structure
VO-Ohpic trihydrate, CAS 476310-60-8
规格 价格/CNY 货期 数量
1 mg ¥ 219 现货
5 mg ¥ 488 现货
10 mg ¥ 763 现货
25 mg ¥ 1,360 现货
50 mg ¥ 2,270 现货
100 mg ¥ 3,730 现货
200 mg ¥ 5,420 现货
500 mg ¥ 8,380 待询
1 mL * 10 mM (in DMSO) ¥ 488 现货
其他形式的 VO-Ohpic trihydrate:
产品目录号及名称: VO-Ohpic trihydrate (T7012)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 VO-Ohpic trihydrate (VO-Ohpic) is a potent inhibitor of PTEN (phosphatase and tensin homolog) with IC50 of 35 nM.
靶点活性 PTEN:35 nM
体外活性 VO-OHpic is a potent small-molecule compound that specifically inhibits PTEN's cellular enzymatic activity, which in turn activates downstream targets such as Akt and FoxO3a. Glucose uptake into adipocytes is dramatically enhanced upon PTEN inhibition with VO-OHpic. PTEN inhibitor accelerates wound healing in fibroblasts[1]. VO-OHpic inhibits cell viability, cell proliferation and colony formation, and induces senescence-associated β-galactosidase activity in Hep3B (low PTEN expression) and to a lesser extent in PLC/PRF/5 (high PTEN expression) cells, but not in PTEN-negative SNU475 cells[2].
体内活性 VO-Ohpic significantly inhibits tumor growth in nude mice bearing xenografts of Hep3B cells[2]. VO-Ohpic administered to C57BL6 mice 30 minutes prior to Kcl-induced asystolic cardiac arrest significantly increases survival, LVPmax and dP/dt max with continued benefit. VO-OHpic also significantly increases lactate clearance and decreases plasma glucose level[3].
激酶实验 VO-OHpic is dissolved in DMSO (100 μM) and diluted further to the required concentration with 1% DMSO. For inhibition studies, PTEN is preincubated with VO-OHpic at RT for 10 min before substrate is added to initialise the reaction. Background absorbance (malachite green assay) and fluorescence (OMFP assay) are determined with VO-OHpic in assay buffer and corrected in the data analysis[1].
细胞实验 Cell proliferation is determined by estimating the amount of bromodeoxyuridine (BrdU) incorporation into DNA by a colorimetric immunoassay. 3×103 cells are cultured in 96-well plates with varying concentrations of VO-OHpic for 72 hours. BrdU is added 24 hours before the end of the treatments. Results are expressed as the percentage inhibition of BrdU incorporation over the control.(Only for Reference)
别名 VO-Ohpic
分子量 415.2
分子式 C12H9N2O8V·3H2O·H
CAS No. 476310-60-8

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 67 mg/mL (161.4 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4085 mL 12.0424 mL 24.0848 mL 60.2119 mL
5 mM 0.4817 mL 2.4085 mL 4.817 mL 12.0424 mL
10 mM 0.2408 mL 1.2042 mL 2.4085 mL 6.0212 mL
20 mM 0.1204 mL 0.6021 mL 1.2042 mL 3.0106 mL
50 mM 0.0482 mL 0.2408 mL 0.4817 mL 1.2042 mL
100 mM 0.0241 mL 0.1204 mL 0.2408 mL 0.6021 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Rosivatz E, et al. ACS Chem Biol. 2006,1(12):780-90. 2. Augello G, et al. Cell Cycle. 2016, 15(4):573-83. 3. Jing Li, et al. American Heart Association Scientific Sessions. 2013.
Valinomycin bpV(phen) SF1670 Ginkgolic acid C17:1 Oroxin B Afzelin BpV(HOpic) Cucurbitacin B

相关化合物库

该产品包含在如下化合物库中:
酪氨酸激酶分子库 代谢化合物库 已知活性化合物库 抗卵巢癌化合物库 氧化还原化合物库 抗肺癌化合物库 抗胰腺癌化合物库 抗肥胖化合物库 抗乳腺癌化合物库 糖酵解化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

VO-Ohpic trihydrate 476310-60-8 Autophagy PI3K/Akt/mTOR signaling PTEN Inhibitor VO-Ohpic VOOhpic trihydrate VO Ohpic trihydrate inhibit VO-Ohpic Trihydrate Phosphatase and tensin homolog MMAC1 inhibitor

 

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