Powder: -20°C for 3 years | In solvent: -80°C for 1 year
UAMC-3203 是一种有效的选择性铁死亡抑制剂,IC50为12 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 189 | 现货 | ||
5 mg | ¥ 428 | 现货 | ||
10 mg | ¥ 688 | 现货 | ||
25 mg | ¥ 1,130 | 现货 | ||
50 mg | ¥ 1,680 | 现货 | ||
100 mg | ¥ 2,530 | 现货 |
产品描述 | UAMC-3203 is a potent and selective Ferroptosis inhibitor (IC50: 12 nM). |
靶点活性 | Ferroptosis:12 nM ( in IMR32 Neuroblastoma cells) |
体外活性 | UAMC-3203 exhibits even lower IC50-values (10 nM in IMR32 Neuroblastoma cells.) combined with an excellent kinetic solubility (> 200 μM). |
体内活性 | In blood plasma of mice having received a daily injection of compound UAMC-3203 over a period of 4 weeks, Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), LDH, creatine kinase (CK), creatinine, urea and troponin T levels were all comparable to background values detected in control mice. |
细胞实验 | In order to determine IC50-values, human neuroblastoma cells (IMR-32) were seeded in a 96-well plate at a density of 25,000 cells/well. The next day, the cells were pretreated for 1h (in triplicates) with a 1/3 dilution series of ferrostatin-1 analogues ranging from 5μM to 0.68nM and Sytox Green (1.6 μM) and a 1/2 dilution series of ferrostatin-1 analogues ranging from 200 nM to 0.78nM and Sytox Green (1.6 μM) for erastin and ferrous ammonium sulphate respectively. After stimulating the cells with erastin (10 μM) or ferrous ammonium sulphate (600 μM), the plate was transferred to a temperature- and CO2-controled FLUOstar Omega fluorescence plate reader. Sytox Green intensity was measured after 13h using an excitation filter of 485 nm and an emission filter of 520 nm. In each setup, Triton-X100 (0.05%) was used to induce lyses of the cells in 6 wells/plate, and was used as 100% cell death reference. The percentage of the cell death was calculated by the formula ((AVG[erastin] - AVG[background]) /(AVG[Triton-X100] - AVG[background])) × 100. Cell death percentage was plotted in GraphPad Prism 6, and IC50-values were calculated using a sigmoidal dose-response (variable slope) curve. |
动物实验 | Vehicle solution (2% DMSO) or compound was administered daily at a concentration of 2 mM (in 0.9% NaCl containing 2% DMSO; 200 μL / 20 g body weight) by intraperitoneal injection. Body temperature and weight were monitored daily. On day 28 the mice were anesthetized with isoflurane and blood was sampled. Hereafter, mice were sacrificed by cervical dislocation. ALT, AST and LDH levels in plasma were measured. CK, creatinine, urea and troponin T were determined. |
别名 | UAMC 3203, UAMC3203 |
分子量 | 471.66 |
分子式 | C25H37N5O2S |
CAS No. | 2271358-64-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 199.3 mM
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.1202 mL | 10.6009 mL | 21.2017 mL | 53.0043 mL |
5 mM | 0.424 mL | 2.1202 mL | 4.2403 mL | 10.6009 mL | |
10 mM | 0.212 mL | 1.0601 mL | 2.1202 mL | 5.3004 mL | |
20 mM | 0.106 mL | 0.53 mL | 1.0601 mL | 2.6502 mL | |
50 mM | 0.0424 mL | 0.212 mL | 0.424 mL | 1.0601 mL | |
100 mM | 0.0212 mL | 0.106 mL | 0.212 mL | 0.53 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
UAMC-3203 2271358-64-4 Apoptosis Ferroptosis UAMC 3203 inhibit Inhibitor UAMC3203 inhibitor